Detailed information of TA system TA153722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/COG3657-dnstrm_HI1420
• Location: 1150468..1151078
• Replicon: plasmid pUW576mp
• Accession: NZ_CP051176
• Organism: Ralstonia solanacearum strain Rs5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A399W208
• Locus tag: HF906_RS21070
• Protein ID: WP_042590727.1
• Coordinates: 1150468..1150776 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HF906_RS21075
• Protein ID: WP_003262836.1
• Coordinates: 1150773..1151078 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF906_RS21045	1146092..1146526	-	435	WP_075453772.1	VOC family protein	-
HF906_RS21050	1146794..1147393	-	600	WP_094394304.1	GNAT family N-acetyltransferase	-
HF906_RS21055	1147527..1148078	+	552	WP_094394302.1	helix-turn-helix transcriptional regulator	-
HF906_RS21060	1148248..1149066	+	819	WP_094394300.1	GntR family transcriptional regulator	-
HF906_RS21065	1149193..1150392	+	1200	WP_094394298.1	MFS transporter	-
HF906_RS21070	1150468..1150776	+	309	WP_042590727.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF906_RS21075	1150773..1151078	+	306	WP_003262836.1	putative addiction module antidote protein	Antitoxin
HF906_RS21080	1151300..1152613	+	1314	WP_141514891.1	metabolite/H+ symporter	-
HF906_RS21085	1152776..1152895	+	120	WP_119447541.1	H-NS histone family protein	-
HF906_RS21090	1153149..1155086	-	1938	WP_193010660.1	helix-hairpin-helix domain-containing protein	-
HF906_RS21095	1155095..1155856	-	762	WP_119447539.1	protein phosphatase 2C domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / fliI / fliM / flgG / flgC / cyaB / cheY / motA / cheW / cheB / cheY / adeG	1..1837383	1837383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-92)

Antitoxin

• Uncharacterized protein HI1420 (9-98)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11655.40 Da
• Isoelectric Point: 7.8889

>T153722 WP_042590727.1 NZ_CP051176:1150468-1150776 [Ralstonia solanacearum]
MLTIRTTELFDDGFCSLRDKAAQRRIQVRIDRLQMGHPGDMKAVRDGIRELRIDHGPGYRLYFVQHGVVLIVLLCGGAKS
TQEADSRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T153722 NZ_CP067059:509720-511045 [Rahnella aceris]
ATGCGTCGTGCGCAGCAGCGTTTAGCAATATGGATGAACGGTATTCCTGTGGGGTACTGGGAAAAGCAAAAAGGCGAGGA
TCGTTTACAGTACTCGCCCGAATGGACAGAAGACTCACAAGGGCGACCTTTGTCGCTTTCCCTGCCGTTTACGCCGGGTA
ATCAGGTGCATCGCGGCAATGTGGTTCGCGATTATTTTGACAATTTACTGCCCGACAGCGAAGGGATCCGCAGGCGCCTG
GCGGTACGTTATCATGCTGAGAGCCTCGAGCCTTTTGATCTGCTGACCGAGTTAGGGCGGGACTGTGTCGGTGCAATTCA
GTTGCTCAATGTCGCGGATCAACCCGAAGGGATATTCACTATCCGCCAGCGTCCGCTTTCAGAACCGGAGATTGCCGGTA
TGCTGCGCAACACGGCGGCAGTTTTGCCGGGCCAGAACAATCAGGACGAAGATTTGCGATTGTCTATCGCTGGCGCACAG
GAAAAAACGGCGCTGTTATGGCACAACGAACAATGGTGTCTGCCTGAGGGAAATACGCCAACCACGCATATTTTTAAACT
ACCGCTGGGTCTGGTGGGCAATATGCAGGCAGACATGAGTACGTCCGTTGAAAACGAATGGCTTTGTTCCCTGATCCTTG
AGCAATATGGAGTGCCAGTTGCTAAAACATACATTGGGCAATTTGAAGATCAAAAAGCGCTGATCGTCGAACGTTTTGAC
CGAAAGCTTTCCTCAGACGAAGCATGGTGGATCCGTTTGCCGCAGGAAGATATGTGTCAGGCCATGGGCGTTTCACCCTT
GCGAAAATATCAGTCGGATGGCGGTCCGGGTATCTCGGATATTATGGAAATCCTGAGCCGATCTGATCAGGCTCAGCAAG
ATCAGTATCAGTTTTTCAGGTCGCAGATAATTTTCTGGTTGATGGCCGCAACTGACGGGCATGCAAAAAATTTCAGTATC
GCGCACCTCTCTCAAAATCGTTATCACATGACACCGCTGTACGATATTTTGTCAGCCTGGCCGGTGATTGGCCGGGGAAA
CAATAAGATCGCATGGCAGAAATGTAAGCTGGCTATGGCCGTCCGTGGCAGCAGTAATTATTACCTGATCAGCAAGATCC
AGCGCAGACACTGGATTAAGCAGGGTGAGCAGAGCGGATTGGGGAAACGACAAATAGAAACCATGATTGATGAAATCATC
GAACTCACACCGGCCGTTATTGAAAATGTCGCGAGGCAATTGTCCAAATCGTTTCCGGCTGAACTGGCTGACGCCATCTT
TACAGGGATGGAACAGCAACGGGTGAGGCTGAAAGCACAGACTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11038.72 Da
• Isoelectric Point: 8.0667

>AT153722 WP_003262836.1 NZ_CP051176:1150773-1151078 [Ralstonia solanacearum]
MTMAQRTLKTRPWDSAEHLRTEADMAAYLNACLEEAGDDPAFITHALGVVARARGMTRLARETGITREGLYKALSEDGNP
SFATMLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT153722 NZ_CP067059:509419-509715 [Rahnella aceris]
ATGAAGACAGTCTATCCGTTAAAAACACTTAACCAGCTCCGTCCGTTACTGGTGGGGTTCCGTAAGGAAAAAGGTCTGAC
GCAAAAGGATGTGTCAGAAAAACTGGGCGTGACCCAGCAGACATATGCCCGACTGGAGGCAAACCCTGCTACTGCCAGCC
TTGACCGGCTGTTTCGGGTATTTAATGTGTTGGGCATTGAGATGGTGTTTTCCTCACAATCCGATCCTGAAAGGCCTTCT
ATGGAGGAAATTAAGCCGGATTATTTAAGTACGCCCGCCAGAAAGGAACAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A399W208