Detailed information of TA system TA153721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 749018..749603
• Replicon: plasmid pUW576mp
• Accession: NZ_CP051176
• Organism: Ralstonia solanacearum strain Rs5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HF906_RS19375
• Protein ID: WP_080894820.1
• Coordinates: 749421..749603 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D8P328
• Locus tag: HF906_RS19370
• Protein ID: WP_013207853.1
• Coordinates: 749018..749410 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF906_RS19355	744895..746700	-	1806	WP_193010855.1	Tar ligand binding domain-containing protein	-
HF906_RS19360	746811..747554	-	744	WP_193010856.1	glycine zipper 2TM domain-containing protein	-
HF906_RS19365	747804..748736	+	933	WP_119447704.1	2-hydroxyacid dehydrogenase	-
HF906_RS19370	749018..749410	-	393	WP_013207853.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HF906_RS19375	749421..749603	-	183	WP_080894820.1	type II toxin-antitoxin system HicA family toxin	Toxin
HF906_RS19380	749532..749723	+	192	WP_193010912.1	hypothetical protein	-
HF906_RS19385	750224..750658	+	435	WP_013207851.1	HIT family protein	-
HF906_RS19390	750677..753871	-	3195	WP_193010857.1	CusA/CzcA family heavy metal efflux RND transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / fliI / fliM / flgG / flgC / cyaB / cheY / motA / cheW / cheB / cheY / adeG	1..1837383	1837383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6622.80 Da
• Isoelectric Point: 11.5522

>T153721 WP_080894820.1 NZ_CP051176:c749603-749421 [Ralstonia solanacearum]
MNSANLIKQLRADGWQLVHVVGSHHQFKHPTKPGKVTVPHPKKDLPPGTVRSILKQAGLK

Nucleotide

• Length: 183 bp

>T153721 NZ_CP067058:174085-174381 [Rahnella aceris]
ATGGAAAAGAAAACTCCACATACGCGCCTTCATATCGTTCAGGGATTTGTTGCAGCAGGAAAGGTGAAAGCCACCAATAC
GGCGCTCAACGGCGCGGATGATTTAGGGCTGACATTTGATGATATGTGCAGTGCGATCCTGGGATTAACGGCAGCTGATT
TTTTTAAAAGTATGACCACCTATAATGATCATACTGTCTGGCAGGACGTATACCGCCCGAAGTTGTCGTGTGGTGACGTA
TATCTAAAACTAACGGTCAGTAACGGTGTTTTGATCGTCTCATTCAAGGAGAAGTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14524.34 Da
• Isoelectric Point: 4.7514

>AT153721 WP_013207853.1 NZ_CP051176:c749410-749018 [Ralstonia solanacearum]
MLYPLYVYVGDAKHAHGVTFPDFPGCHAAADEWEDLPRAVQEAAEAHFAGDDDPIPAPTPLERLTADPEYEGGVWMLFNL
DLSRLRSRTVRINVSVPEGLLSQIDAFAQRRHMTRSAFLALAAQHEMEHV

Nucleotide

• Length: 393 bp

>AT153721 NZ_CP067058:174383-174784 [Rahnella aceris]
ATGAAATGTTCCGTTTGTGGCGGTGCAGAACTTGAGTTTGGTATCAAAAAAGTCCCTTACTCATTCCGTGGCCGTAAAAC
AGTTATCGAGGCAGAAGGTGACCACTGTCCGGCGTGTGGTGAAGTCGTGATGGATCGTGAGCAGTCTATGGCCTACATGG
CAAAGGTGAAGTCATTTAAGGCCAAAGTCATCGCGGAAACCATCGAACCTGAGTACATCATTCAGGTGCGTAAAAAACTT
GAACTCACGCAGAAAGAGGCCAGCGAGATTTTTGGCGGGGGCGTGAATGCCTTTTCGCGTTATGAAAAGGGTAAGGCACA
GCCACACCCTTCGATCATCAAACTCCTGCGCGTACTCGATCACCATCCCGAGTTATTGGATGAAGTGCGCAAACAGGCAT
AA

Structures

Antitoxin

• AlphaFold DB: A0A399W790