Detailed information of TA system TA153718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 1516273..1516883
• Replicon: plasmid pUW763mp
• Accession: NZ_CP051174
• Organism: Ralstonia solanacearum strain UW763

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HF907_RS23320
• Protein ID: WP_064478232.1
• Coordinates: 1516273..1516581 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S4U6E5
• Locus tag: HF907_RS23325
• Protein ID: WP_064478231.1
• Coordinates: 1516578..1516883 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF907_RS23290	1511552..1511848	+	297	Protein_1166	hypothetical protein	-
HF907_RS23295	1512121..1512480	-	360	Protein_1167	recombinase family protein	-
HF907_RS23300	1512589..1513188	-	600	WP_064478235.1	GNAT family N-acetyltransferase	-
HF907_RS23305	1513321..1513872	+	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
HF907_RS23310	1514050..1514871	+	822	WP_064478234.1	GntR family transcriptional regulator	-
HF907_RS23315	1514998..1516197	+	1200	WP_064478233.1	MFS transporter	-
HF907_RS23320	1516273..1516581	+	309	WP_064478232.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF907_RS23325	1516578..1516883	+	306	WP_064478231.1	putative addiction module antidote protein	Antitoxin
HF907_RS23330	1517096..1518409	+	1314	WP_064478230.1	metabolite/H+ symporter	-
HF907_RS23335	1518514..1519182	-	669	WP_038938024.1	hypothetical protein	-
HF907_RS23340	1519349..1519566	-	218	Protein_1176	IS5/IS1182 family transposase	-
HF907_RS23345	1519871..1520155	+	285	WP_011003391.1	hypothetical protein	-
HF907_RS23350	1520286..1520567	+	282	WP_028861300.1	hypothetical protein	-
HF907_RS23355	1520886..1521278	-	393	WP_064478229.1	RidA family protein	-
HF907_RS23360	1521306..1521716	-	411	WP_016722332.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG / htpB / acrB / fliI / fliG / fliM / flgG / flgC / cyaB / motA / cheW / cheB / cheY	1..2021285	2021285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11864.65 Da
• Isoelectric Point: 7.2461

>T153718 WP_064478232.1 NZ_CP051174:1516273-1516581 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGHPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T153718 NZ_CP067058:29066-29398 [Rahnella aceris]
ATGGTTTCCCGTTTTGTACCTGACTCGGGGGATTTGATCTGGATTGATTTTGATCCTGTTGCGGGACACGAGCAAGGAGG
GCATCGTCCTGCCGTTGTCCTCAGTCCTTTTGCTTATAACAACAAAGTGGGGCTTTTACTCTGTGTTCCCTGTACGACAC
AAGTCAAAAACTACCCGTTCGAAGTGGAATTATCAGGAAGCCGTGAGGGTGTCGCCCTAGCCGATCAGGTGACCAGTGTT
GACTGGCGAGCCAGAAAGATAACCCAAAAAGGAACGGTTGCTGTAAACGAGTTGGCTGAAATACGTGCCAAGGCTAAATC
TCTTATAGGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.62 Da
• Isoelectric Point: 7.2780

>AT153718 WP_064478231.1 NZ_CP051174:1516578-1516883 [Ralstonia solanacearum]
MTMAQRTIKTRLWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT153718 NZ_CP067058:28821-29066 [Rahnella aceris]
ATGGCACAAGTTATCGTAAAAAAATGGGGCAATAGCCCGTCAGTCAGGCTACCGGTTGCAATCATGAAAGCAGCCTCTCT
GAACATTGATGATACCGTTGAAATTGTCGTGGAAGAAGGACGCATCATCATCGTTCCAACGAGAGTAAAAGAATACTCGC
TGGATGCTCTTCTGGCGGGGATCTCTGCTGATAATATCCATGATAAAGCCGATTTTGGTGCTCCGACAGGCAAGGAATTA
CTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4U6E5