Detailed information of TA system TA153712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2926992..2927653
• Replicon: chromosome
• Accession: NZ_CP051171
• Organism: Ralstonia solanacearum strain RUN2279

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HF908_RS13665
• Protein ID: WP_193026209.1
• Coordinates: 2927240..2927653 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D8N5P4
• Locus tag: HF908_RS13660
• Protein ID: WP_020749302.1
• Coordinates: 2926992..2927243 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF908_RS13650	2922415..2922810	-	396	WP_193026207.1	hypothetical protein	-
HF908_RS13655	2922816..2926916	-	4101	WP_193026208.1	tape measure protein	-
HF908_RS13660	2926992..2927243	+	252	WP_020749302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HF908_RS13665	2927240..2927653	+	414	WP_193026209.1	type II toxin-antitoxin system VapC family toxin	Toxin
HF908_RS13670	2927798..2928946	-	1149	WP_193026210.1	DNA cytosine methyltransferase	-
HF908_RS13675	2929498..2929788	+	291	WP_193026211.1	H-NS histone family protein	-
HF908_RS13680	2929831..2930064	-	234	WP_193026212.1	hypothetical protein	-
HF908_RS13685	2930312..2931775	+	1464	WP_193026213.1	YOPP/AvrRxv family protein	-
HF908_RS13690	2931970..2932260	+	291	WP_064051869.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2913270..2975871	62601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15742.09 Da
• Isoelectric Point: 6.2337

>T153712 WP_193026209.1 NZ_CP051171:2927240-2927653 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKQPDARVLAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLETELPNYFLGRLLDVD
AHTADRWGRLMSSANRPLPAIDGLLAATALQHDLILVTRNTKDFVGLDVPLINPWET

Nucleotide

• Length: 414 bp

>T153712 NZ_CP067057:c1036449-1036135 [Rahnella aceris]
ATGAACGATATGCTCAGGATAAGCAGTTTCCGCGATCAATGGCTGGAAGACTTTTTCCACTTCGCCACTCCCCATCGCAA
AATACCCTGGACGCTGGCAACTGCTCTGGAAAGGAAGCTGGATATGATCAATGCCGCAATGGATTATCGCGATTTACTCT
GGCCCCCGGGCAATCGCTATGAAGATCTGGCCCCCCCGTTAAAGGGATTTTCATCGATCAGGGTGAATGAGCAGTACCGT
CTGATTTTTTACTGGCGGGAAGGAAAAGCACATGGCCTGTATTTAGATCCCCACGGTTACAGGAGTTACCGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9258.46 Da
• Isoelectric Point: 5.0628

>AT153712 WP_020749302.1 NZ_CP051171:2926992-2927243 [Ralstonia solanacearum]
MQSWQLQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGSGESLVDFMRQSPLAGLDDVVFERERSLPRE
VDF

Nucleotide

• Length: 252 bp

>AT153712 NZ_CP067057:c1036081-1035764 [Rahnella aceris]
ATGGCTGTGACCACACGTAAACCCGCTACTGTTGGTGATATTTTACTTTACGAATTTCTCGAGCCTGCAGGCCTGCGGAT
CAACGATCTCGCCGACATGCTGAATGTTCACCGCAATACCATCAGCGCGCTGGTGAATAACAACCGCAAACTGACCGTTG
ATATTGCCTTCCGGCTGGCAAAGACATTTGAGACCTCTGTCGAGTTTTGGTTAAACCTGCAAACCAATGTCGATATTTGG
GAAGTGCGCAATAACCCGCGCCTGATGGATGACATTAACAAAGTCGCCCATCTGAAAGATTTTATGAAAAAGTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A5B7U0Z6