Detailed information of TA system TA153709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 426907..427540
• Replicon: chromosome
• Accession: NZ_CP051171
• Organism: Ralstonia solanacearum strain RUN2279

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HF908_RS02010
• Protein ID: WP_193027353.1
• Coordinates: 427127..427540 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D8N529
• Locus tag: HF908_RS02005
• Protein ID: WP_020747258.1
• Coordinates: 426907..427140 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF908_RS01990	422486..423781	-	1296	WP_193027348.1	MFS transporter	-
HF908_RS01995	423789..425021	-	1233	WP_193027350.1	AGE family epimerase/isomerase	-
HF908_RS02000	425243..426850	+	1608	WP_193028416.1	glucan biosynthesis protein	-
HF908_RS02005	426907..427140	+	234	WP_020747258.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HF908_RS02010	427127..427540	+	414	WP_193027353.1	PIN domain-containing protein	Toxin
HF908_RS02015	427515..428144	-	630	WP_193027355.1	DUF1415 family protein	-
HF908_RS02020	428188..429684	-	1497	WP_193027357.1	glycerol kinase GlpK	-
HF908_RS02025	429852..430079	-	228	Protein_402	carbohydrate ABC transporter substrate-binding protein	-
HF908_RS26130	430080..430202	-	123	Protein_403	ABC transporter	-
HF908_RS02030	430459..432036	-	1578	WP_193027360.1	glycerol-3-phosphate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15091.41 Da
• Isoelectric Point: 5.5407

>T153709 WP_193027353.1 NZ_CP051171:427127-427540 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADAAKADTAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRIEPLTLET
HELGRRLAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVVEQHLRIVNPFSARA

Nucleotide

• Length: 414 bp

>T153709 NZ_CP067046:c109820-109377 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8806.98 Da
• Isoelectric Point: 8.7287

>AT153709 WP_020747258.1 NZ_CP051171:426907-427140 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRSARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT153709 NZ_CP067046:c110047-109817 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4TW23