Detailed information of TA system TA153703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 419454..420087
• Replicon: chromosome
• Accession: NZ_CP051169
• Organism: Ralstonia solanacearum strain RUN2474

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HF909_RS01990
• Protein ID: WP_193027353.1
• Coordinates: 419674..420087 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D8N529
• Locus tag: HF909_RS01985
• Protein ID: WP_020747258.1
• Coordinates: 419454..419687 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF909_RS01970	415033..416328	-	1296	WP_193035687.1	MFS transporter	-
HF909_RS01975	416336..417568	-	1233	WP_193027350.1	AGE family epimerase/isomerase	-
HF909_RS01980	417790..419397	+	1608	WP_193028416.1	glucan biosynthesis protein	-
HF909_RS01985	419454..419687	+	234	WP_020747258.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HF909_RS01990	419674..420087	+	414	WP_193027353.1	PIN domain-containing protein	Toxin
HF909_RS01995	420062..420691	-	630	WP_193027355.1	DUF1415 family protein	-
HF909_RS02000	420735..422231	-	1497	WP_193027357.1	glycerol kinase GlpK	-
HF909_RS02005	422399..422626	-	228	Protein_398	carbohydrate ABC transporter substrate-binding protein	-
HF909_RS25930	422627..422749	-	123	Protein_399	ABC transporter	-
HF909_RS02010	423006..424583	-	1578	WP_193027360.1	glycerol-3-phosphate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15091.41 Da
• Isoelectric Point: 5.5407

>T153703 WP_193027353.1 NZ_CP051169:419674-420087 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADAAKADTAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRIEPLTLET
HELGRRLAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVVEQHLRIVNPFSARA

Nucleotide

• Length: 414 bp

>T153703 NZ_CP067045:c3956652-3956335 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8806.98 Da
• Isoelectric Point: 8.7287

>AT153703 WP_020747258.1 NZ_CP051169:419454-419687 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRSARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT153703 NZ_CP067045:c3956342-3956055 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S4TW23