Detailed information of TA system TA153687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2529831..2530360
• Replicon: chromosome
• Accession: NZ_CP051165
• Organism: Staphylococcus aureus strain NGA84b

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HF584_RS12170
• Protein ID: WP_000621175.1
• Coordinates: 2529998..2530360 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HF584_RS12165
• Protein ID: WP_000948331.1
• Coordinates: 2529831..2530001 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF584_RS12135 (2524867)	2524867..2525427	+	561	WP_054190433.1	K(+)-transporting ATPase subunit C	-
HF584_RS12140 (2525636)	2525636..2526115	+	480	WP_054190432.1	hypothetical protein	-
HF584_RS12145 (2526108)	2526108..2527691	+	1584	WP_054190431.1	PH domain-containing protein	-
HF584_RS12150 (2527678)	2527678..2528169	+	492	WP_054190430.1	PH domain-containing protein	-
HF584_RS12155 (2528173)	2528173..2528532	+	360	WP_000581197.1	holo-ACP synthase	-
HF584_RS12160 (2528598)	2528598..2529746	+	1149	WP_054190429.1	alanine racemase	-
HF584_RS12165 (2529831)	2529831..2530001	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HF584_RS12170 (2529998)	2529998..2530360	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HF584_RS12175 (2530710)	2530710..2531711	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HF584_RS12180 (2531830)	2531830..2532156	+	327	WP_001052491.1	anti-sigma factor antagonist	-
HF584_RS12185 (2532158)	2532158..2532637	+	480	WP_054190427.1	anti-sigma B factor RsbW	-
HF584_RS12190 (2532612)	2532612..2533382	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T153687 WP_000621175.1 NZ_CP051165:2529998-2530360 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T153687 NZ_CP067032:613824-614183 [Agrobacterium fabrum]
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTCTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT153687 WP_000948331.1 NZ_CP051165:2529831-2530001 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT153687 NZ_CP067032:613558-613824 [Agrobacterium fabrum]
ATGACCGTGACCACGAAAATAAGACGCCAGGGCGGCGCGGCGGTGATGACGATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAGCAACTGACGCTCGAGGTCGATAATGGCGCTCTGGTGGCAAGCCCGGTTCGACTGGAAAAGAAAC
GCTTCACGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCG
CCTCCTGTCGGCAAAGAAGCGTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1