Detailed information of TA system TA153686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2510105..2510619
• Replicon: chromosome
• Accession: NZ_CP051165
• Organism: Staphylococcus aureus strain NGA84b

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: HF584_RS12080
• Protein ID: WP_054190442.1
• Coordinates: 2510410..2510619 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: HF584_RS12075
• Protein ID: WP_000138934.1
• Coordinates: 2510105..2510398 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF584_RS12055 (2505597)	2505597..2506238	+	642	WP_054190444.1	thiamine phosphate synthase	-
HF584_RS12060 (2506324)	2506324..2507196	+	873	WP_000725800.1	membrane protein insertase YidC	-
HF584_RS12065 (2507776)	2507776..2508423	-	648	WP_001187616.1	HD domain-containing protein	-
HF584_RS12070 (2508451)	2508451..2509935	-	1485	WP_054190443.1	cardiolipin synthase	-
HF584_RS12075 (2510105)	2510105..2510398	+	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
HF584_RS12080 (2510410)	2510410..2510619	+	210	WP_054190442.1	heavy metal-associated domain-containing protein	Toxin
HF584_RS12085 (2510747)	2510747..2510884	+	138	WP_000828354.1	Lmo0850 family protein	-
HF584_RS12090 (2511148)	2511148..2512350	-	1203	WP_054190441.1	FtsW/RodA/SpoVE family cell cycle protein	-
HF584_RS12095 (2512668)	2512668..2513738	+	1071	WP_054190440.1	D-alanine--D-alanine ligase	-
HF584_RS12100 (2513753)	2513753..2515111	+	1359	WP_054190439.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7830.77 Da
• Isoelectric Point: 4.1008

>T153686 WP_054190442.1 NZ_CP051165:2510410-2510619 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISSMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKILF

Nucleotide

• Length: 210 bp

>T153686 NZ_CP067032:c609067-608732 [Agrobacterium fabrum]
GTGACGAATAAGAAAGATCACGGAAAAGACGCGGCATTGAAGCGAGCCACCCTGCCGCGCAGGTCGGATTTCACCAAGCA
ATTTATCAAAGACTGGCAACGGCTGAACAATTCCGGGCGTTACGACATGGTGAGACTGAAGGAGATCATGCTCCTTCTTA
TCGCCAACGGCGCGCCGTTACCGACGCAGTTTCGTGACCATGAACTGACCGGAGACTGGCGCGATCACCGGGAATGCCAT
GTCGGCGGGGATTTTCTGTTGATCTACACAGTCGATGAGAAGCAGAACCTTCTCATCTTCACCCGCGCGGGAACGCACGC
CGAGCTTTTCAGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT153686 WP_000138934.1 NZ_CP051165:2510105-2510398 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT153686 NZ_CP067032:c609339-609064 [Agrobacterium fabrum]
ATGACCGCAAATGCTTATGTAAGAGCGCGCATCGATCAGACACTCAAGGACGACGCGACGGCCGTGCTGGACCGCCTGGG
ACTGACTGTTTCAGATGTCATGCGAATGATGCTGACGCGCATTGCCCGAGAAAAAGCTCTGCCGATAGAACTGACACAAC
CAAACGCTGAAACCCTTGCCGCGATTGAAGAAGCGCGCGCCATCGCGGCGGCCGGGCGCAATCGTTTCGGCACGTCTGAA
GCCTTGTTTGAGGCGCTGGACGCTGGCAAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0D1GBN9