Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 676628..677264 Replicon chromosome
Accession NZ_CP051162
Organism Geobacillus subterraneus strain CPW16

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag HF500_RS03355 Protein ID WP_003253417.1
Coordinates 676914..677264 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID S5ZZQ7
Locus tag HF500_RS03350 Protein ID WP_020958454.1
Coordinates 676628..676909 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF500_RS03330 672807..673424 - 618 WP_063166927.1 rhomboid family intramembrane serine protease -
HF500_RS03335 673574..673963 + 390 WP_063166926.1 holo-ACP synthase -
HF500_RS03340 674043..675056 + 1014 WP_063166925.1 outer membrane lipoprotein carrier protein LolA -
HF500_RS03345 675346..676500 + 1155 WP_063166924.1 alanine racemase -
HF500_RS03350 676628..676909 + 282 WP_020958454.1 hypothetical protein Antitoxin
HF500_RS03355 676914..677264 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HF500_RS03360 677597..678844 + 1248 WP_168368019.1 transposase -
HF500_RS03365 679019..681181 + 2163 WP_168368020.1 RNA-binding transcriptional accessory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T153671 WP_003253417.1 NZ_CP051162:676914-677264 [Geobacillus subterraneus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T153671 NZ_CP067022:1722543-1722671 [Pseudomonas cannabina pv. alisalensis]
ATGGTCTACGTTCTGCACTGTTTTCAGAAAAAGTCGACTTCGGGTATTAAAACGGCAAAACATGATATTGAATTGATCAA
ATCACGACTGAAAGCTGCACAAGCACACGCAGAGAGTAATAGACGATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10641.11 Da        Isoelectric Point: 7.2027

>AT153671 WP_020958454.1 NZ_CP051162:676628-676909 [Geobacillus subterraneus]
VSESGATTEIIVRLPQSLLTELDGLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT153671 NZ_CP067022:1722668-1722988 [Pseudomonas cannabina pv. alisalensis]
ATGATTGATATCGAAGAAGGTAGCGGCAACGTTTACGCTGATCTTGGGCGAGCTGATGCGAATGAAATGTCAATCAAGGC
TCAGTTAGCCTCAAAAATCGGCACCATCATCAAGGCCCGACACCTGACCCAGCTTCAGGCAGCTGAGATACTGGCAATGC
CTCAGCCGAAGGTTTCAGAGATGCTTAGAGGCAAGTTCAGAGGCATAAGCGAGGCTAAAATGATGGACTGTCTTTCGCGT
CTCGGGCGTGACGTGCAGATCGTGGTTAAGGCAGCTCCTCGTTCTCGTCGAGAGGGGCGAGTTGAAGTTGTATTTGCTTA
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A143ML40

References