Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5331895..5332520 Replicon chromosome
Accession NZ_CP051160
Organism Klebsiella pneumoniae strain KP20191015

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HF573_RS26420 Protein ID WP_002882817.1
Coordinates 5331895..5332278 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HF573_RS26425 Protein ID WP_004150355.1
Coordinates 5332278..5332520 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF573_RS26405 5329261..5330163 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HF573_RS26410 5330160..5330795 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HF573_RS26415 5330792..5331721 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HF573_RS26420 5331895..5332278 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HF573_RS26425 5332278..5332520 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HF573_RS26430 5332725..5333642 + 918 WP_002882812.1 alpha/beta hydrolase -
HF573_RS26435 5333656..5334597 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HF573_RS26440 5334642..5335079 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HF573_RS26445 5335076..5335936 - 861 WP_002882807.1 virulence factor BrkB family protein -
HF573_RS26450 5335930..5336529 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153651 WP_002882817.1 NZ_CP051160:c5332278-5331895 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153651 NZ_CP067004:c4656957-4656805 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153651 WP_004150355.1 NZ_CP051160:c5332520-5332278 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153651 NZ_CP067004:c4656725-4656591 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References