Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 329042..329685 Replicon chromosome
Accession NZ_CP051160
Organism Klebsiella pneumoniae strain KP20191015

Toxin (Protein)


Gene name vagD Uniprot ID R4IT02
Locus tag HF573_RS01520 Protein ID WP_016338372.1
Coordinates 329042..329458 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag HF573_RS01525 Protein ID WP_016338373.1
Coordinates 329455..329685 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF573_RS01505 324090..326198 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
HF573_RS01510 326188..327465 - 1278 WP_016338369.1 HlyD family secretion protein -
HF573_RS01515 328124..329001 + 878 Protein_299 restriction endonuclease -
HF573_RS01520 329042..329458 - 417 WP_016338372.1 type II toxin-antitoxin system VapC family toxin Toxin
HF573_RS01525 329455..329685 - 231 WP_016338373.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HF573_RS01530 330206..330526 + 321 WP_004197770.1 hypothetical protein -
HF573_RS01535 330582..330890 + 309 WP_032431398.1 hypothetical protein -
HF573_RS01540 330918..331247 + 330 WP_004197773.1 hypothetical protein -
HF573_RS01545 331296..332114 + 819 WP_004197776.1 hypothetical protein -
HF573_RS01550 332114..332896 + 783 WP_004197768.1 site-specific integrase -
HF573_RS01555 333063..334025 + 963 WP_004197772.1 Abi family protein -
HF573_RS01560 334182..334655 - 474 WP_047353183.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage aph(3')-Ia - 316711..368728 52017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15060.57 Da        Isoelectric Point: 9.2738

>T153633 WP_016338372.1 NZ_CP051160:c329458-329042 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T153633 NZ_CP066997:c1332358-1331867 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGTGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT153633 WP_016338373.1 NZ_CP051160:c329685-329455 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT153633 NZ_CP066997:c1332645-1332355 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4IT02

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References