Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3363987..3364612 Replicon chromosome
Accession NZ_CP051159
Organism Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HF574_RS16925 Protein ID WP_002882817.1
Coordinates 3364229..3364612 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HF574_RS16920 Protein ID WP_004150355.1
Coordinates 3363987..3364229 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF574_RS16895 3359978..3360577 + 600 WP_004151865.1 glucose-1-phosphatase -
HF574_RS16900 3360571..3361431 + 861 WP_002882807.1 virulence factor BrkB family protein -
HF574_RS16905 3361428..3361865 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HF574_RS16910 3361910..3362851 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HF574_RS16915 3362865..3363782 - 918 WP_002882812.1 alpha/beta hydrolase -
HF574_RS16920 3363987..3364229 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HF574_RS16925 3364229..3364612 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HF574_RS16930 3364786..3365715 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HF574_RS16935 3365712..3366347 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HF574_RS16940 3366344..3367246 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153628 WP_002882817.1 NZ_CP051159:3364229-3364612 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153628 NZ_CP066995:c4671346-4671212 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153628 WP_004150355.1 NZ_CP051159:3363987-3364229 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153628 NZ_CP066995:c4671578-4671426 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References