Detailed information of TA system TA153625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 2532437..2533107
• Replicon: chromosome
• Accession: NZ_CP051159
• Organism: Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HF574_RS12790
• Protein ID: WP_004213072.1
• Coordinates: 2532664..2533107 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HF574_RS12785
• Protein ID: WP_004213073.1
• Coordinates: 2532437..2532667 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF574_RS12760	2528660..2529559	-	900	WP_004225022.1	nucleotide-binding protein	-
HF574_RS12765	2529549..2529839	-	291	WP_004213078.1	hypothetical protein	-
HF574_RS12770	2530191..2530397	+	207	WP_004213077.1	hypothetical protein	-
HF574_RS12775	2530387..2530680	-	294	WP_004213076.1	hypothetical protein	-
HF574_RS12780	2530696..2531829	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HF574_RS12785	2532437..2532667	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HF574_RS12790	2532664..2533107	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HF574_RS12795	2533121..2533537	-	417	Protein_2514	restriction endonuclease	-
HF574_RS12800	2533541..2533834	-	294	Protein_2515	transposase	-
HF574_RS12805	2534251..2534885	+	635	Protein_2516	response regulator	-
HF574_RS12810	2535400..2535806	-	407	Protein_2517	GAF domain-containing protein	-
HF574_RS12815	2535897..2536817	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
HF574_RS12820	2536866..2537357	-	492	WP_011251327.1	DM13 domain-containing protein	-
HF574_RS12825	2537420..2537695	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T153625 WP_004213072.1 NZ_CP051159:2532664-2533107 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T153625 NZ_CP066994:361472-361606 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT153625 WP_004213073.1 NZ_CP051159:2532437-2532667 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT153625 NZ_CP066994:361240-361392 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620