Detailed information of TA system TA153610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4007650..4008288
• Replicon: chromosome
• Accession: NZ_CP051158
• Organism: Escherichia coli strain 17MR471

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HF575_RS18955
• Protein ID: WP_000813794.1
• Coordinates: 4008112..4008288 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HF575_RS18950
• Protein ID: WP_001270286.1
• Coordinates: 4007650..4008066 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF575_RS18930	4002802..4003743	-	942	WP_001251319.1	ABC transporter permease	-
HF575_RS18935	4003744..4004757	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
HF575_RS18940	4004775..4005920	-	1146	WP_176607795.1	ABC transporter substrate-binding protein	-
HF575_RS18945	4006162..4007571	-	1410	WP_000760625.1	PLP-dependent aminotransferase family protein	-
HF575_RS18950	4007650..4008066	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HF575_RS18955	4008112..4008288	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HF575_RS18960	4008510..4008740	+	231	WP_000494244.1	YncJ family protein	-
HF575_RS18965	4008832..4010793	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HF575_RS18970	4010866..4011402	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HF575_RS18975	4011455..4012669	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T153610 WP_000813794.1 NZ_CP051158:c4008288-4008112 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T153610 NZ_CP066988:3797112-3797603 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT153610 WP_001270286.1 NZ_CP051158:c4008066-4007650 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT153610 NZ_CP066988:3796825-3797115 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Structures

Toxin

• PDB: 6HPB