Detailed information of TA system TA153609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3917308..3918896
• Replicon: chromosome
• Accession: NZ_CP051158
• Organism: Escherichia coli strain 17MR471

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: HF575_RS18580
• Protein ID: WP_001125439.1
• Coordinates: 3917574..3918896 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HF575_RS18575
• Protein ID: WP_001301023.1
• Coordinates: 3917308..3917574 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF575_RS18570	3912446..3917085	+	4640	Protein_3605	autotransporter barrel domain-containing lipoprotein	-
HF575_RS18575	3917308..3917574	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HF575_RS18580	3917574..3918896	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HF575_RS18585	3919184..3920131	-	948	WP_127662173.1	hypothetical protein	-
HF575_RS18590	3920943..3921302	-	360	WP_063078926.1	hypothetical protein	-
HF575_RS18595	3921320..3922258	-	939	WP_127662227.1	hypothetical protein	-
HF575_RS18600	3922653..3922861	+	209	Protein_3611	helix-turn-helix transcriptional regulator	-
HF575_RS18605	3922990..3923355	+	366	Protein_3612	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T153609 WP_001125439.1 NZ_CP051158:3917574-3918896 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T153609 NZ_CP066987:c1177705-1177214 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT153609 WP_001301023.1 NZ_CP051158:3917308-3917574 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT153609 NZ_CP066987:c1177992-1177702 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU