Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3853077..3853603 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HF575_RS18215 Protein ID WP_000323025.1
Coordinates 3853316..3853603 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HF575_RS18210 Protein ID WP_000534858.1
Coordinates 3853077..3853316 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF575_RS18180 3848653..3849075 + 423 WP_001151262.1 DUF977 family protein -
HF575_RS18185 3849072..3849419 + 348 WP_096264793.1 class I SAM-dependent methyltransferase -
HF575_RS18190 3849430..3850127 - 698 Protein_3529 IS1 family transposase -
HF575_RS18195 3850435..3851775 + 1341 WP_000589013.1 ISNCY family transposase -
HF575_RS18200 3852212..3852544 - 333 WP_001301033.1 protein FlxA -
HF575_RS18205 3852747..3853052 - 306 WP_001326990.1 hypothetical protein -
HF575_RS18210 3853077..3853316 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HF575_RS18215 3853316..3853603 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HF575_RS18220 3853675..3854718 + 1044 WP_096264855.1 DUF968 domain-containing protein -
HF575_RS18225 3854732..3855484 + 753 WP_001047135.1 antitermination protein -
HF575_RS18230 3855762..3855851 - 90 WP_122987542.1 hypothetical protein -
HF575_RS18235 3855906..3856118 - 213 WP_024172454.1 cold shock-like protein CspF -
HF575_RS18240 3856417..3856632 + 216 WP_000066485.1 cold shock-like protein CspB -
HF575_RS18245 3857386..3857469 + 84 Protein_3540 holin -
HF575_RS18250 3857584..3857799 + 216 WP_000839590.1 phage lysis protein EssD -
HF575_RS18255 3857804..3858115 + 312 WP_000189915.1 YdfR family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3819280..3878662 59382


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T153608 WP_000323025.1 NZ_CP051158:3853316-3853603 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T153608 NZ_CP066987:334969-335103 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT153608 WP_000534858.1 NZ_CP051158:3853077-3853316 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT153608 NZ_CP066987:334740-334892 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References