Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2420566..2421220 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HF575_RS11380 Protein ID WP_000244777.1
Coordinates 2420813..2421220 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HF575_RS11375 Protein ID WP_000354046.1
Coordinates 2420566..2420832 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF575_RS11350 2415735..2416478 + 744 WP_000951964.1 SDR family oxidoreductase -
HF575_RS11355 2416535..2417968 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HF575_RS11360 2418013..2418324 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HF575_RS11365 2418488..2419147 + 660 WP_000250274.1 hemolysin III family protein -
HF575_RS11370 2419343..2420323 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HF575_RS11375 2420566..2420832 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HF575_RS11380 2420813..2421220 + 408 WP_000244777.1 toxin CptA Toxin
HF575_RS11385 2421260..2421781 - 522 WP_001055874.1 flavodoxin FldB -
HF575_RS11390 2421893..2422789 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HF575_RS11395 2422814..2423524 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HF575_RS11400 2423530..2425263 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T153597 WP_000244777.1 NZ_CP051158:2420813-2421220 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T153597 NZ_CP066984:154439-154591 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT153597 WP_000354046.1 NZ_CP051158:2420566-2420832 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153597 NZ_CP066984:154671-154805 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References