Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 828147..828723 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HF575_RS03900 Protein ID WP_001297643.1
Coordinates 828436..828723 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HF575_RS03895 Protein ID WP_000063156.1
Coordinates 828147..828449 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF575_RS03880 824743..826893 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HF575_RS03885 826943..827146 + 204 WP_000467859.1 YbdD/YjiX family protein -
HF575_RS03890 827157..828113 + 957 WP_001297640.1 GTPase -
HF575_RS03895 828147..828449 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HF575_RS03900 828436..828723 - 288 WP_001297643.1 BrnT family toxin Toxin
HF575_RS03905 828922..829619 - 698 Protein_753 IS1 family transposase -
HF575_RS03910 829676..829771 + 96 Protein_754 dienelactone hydrolase family protein -
HF575_RS03915 829917..831149 + 1233 WP_053885127.1 multidrug efflux MFS transporter MdtM -
HF575_RS03920 831190..832470 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T153591 WP_001297643.1 NZ_CP051158:c828723-828436 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T153591 NZ_CP066982:353234-353386 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT153591 WP_000063156.1 NZ_CP051158:c828449-828147 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT153591 NZ_CP066982:353466-353600 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References