Detailed information of TA system TA153569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/FR47-DUF1778
• Location: 93529..94326
• Replicon: plasmid pAMS-38a
• Accession: NZ_CP051133
• Organism: Enterobacter hormaechei strain AMS-38

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: F5S3R9
• Locus tag: HDB52_RS23995
• Protein ID: WP_006812498.1
• Coordinates: 93529..94053 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: HDB52_RS24000
• Protein ID: WP_168728690.1
• Coordinates: 94057..94326 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HDB52_RS23955	89243..90409	+	1167	Protein_103	phage tail protein	-
HDB52_RS23960	90411..90842	+	432	WP_042863549.1	tail fiber assembly protein	-
HDB52_RS23965	91217..91918	+	702	Protein_105	phage tail sheath subtilisin-like domain-containing protein	-
HDB52_RS23970	91893..91988	+	96	Protein_106	receptor-recognizing protein	-
HDB52_RS23975	92063..92386	+	324	WP_042863551.1	hypothetical protein	-
HDB52_RS23980	92400..93092	+	693	WP_006812500.1	membrane protein	-
HDB52_RS23985	93094..93345	+	252	WP_042863552.1	hypothetical protein	-
HDB52_RS23990	93296..93502	+	207	WP_023316023.1	hypothetical protein	-
HDB52_RS23995	93529..94053	-	525	WP_006812498.1	GNAT family N-acetyltransferase	Toxin
HDB52_RS24000	94057..94326	-	270	WP_168728690.1	DUF1778 domain-containing protein	Antitoxin
HDB52_RS24005	94627..95292	+	666	WP_023315962.1	AAA family ATPase	-
HDB52_RS24010	95298..95651	+	354	WP_160859104.1	hypothetical protein	-
HDB52_RS24015	95692..96489	-	798	WP_077990606.1	DUF2971 domain-containing protein	-
HDB52_RS24020	96753..97478	+	726	WP_100229815.1	hypothetical protein	-
HDB52_RS24025	97539..98879	+	1341	WP_006812582.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108999	108999

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19356.13 Da
• Isoelectric Point: 8.9844

>T153569 WP_006812498.1 NZ_CP051133:c94053-93529 [Enterobacter hormaechei]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Nucleotide

• Length: 525 bp

>T153569 NZ_CP066970:c4676102-4675968 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10042.45 Da
• Isoelectric Point: 5.1299

>AT153569 WP_168728690.1 NZ_CP051133:c94326-94057 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSMG

Nucleotide

• Length: 270 bp

>AT153569 NZ_CP066970:c4676331-4676179 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Structures

Toxin

• AlphaFold DB: A0A2J0QWY8