Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 173892..174534 Replicon chromosome
Accession NZ_CP051013
Organism Bacillus tropicus strain LM1212-DB

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag HCM98_RS01005 Protein ID WP_000635963.1
Coordinates 174184..174534 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HCM98_RS01000 Protein ID WP_000004570.1
Coordinates 173892..174179 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCM98_RS00975 (HCM98_00975) 169058..170020 + 963 WP_029439196.1 UV DNA damage repair endonuclease UvsE -
HCM98_RS00980 (HCM98_00980) 170188..170736 - 549 WP_029439197.1 rhomboid family intramembrane serine protease -
HCM98_RS00985 (HCM98_00985) 170829..171188 + 360 WP_000635047.1 holo-ACP synthase -
HCM98_RS00990 (HCM98_00990) 171345..172295 + 951 WP_029141035.1 outer membrane lipoprotein carrier protein LolA -
HCM98_RS00995 (HCM98_00995) 172414..173583 + 1170 WP_029439198.1 alanine racemase -
HCM98_RS01000 (HCM98_01000) 173892..174179 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HCM98_RS01005 (HCM98_01005) 174184..174534 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HCM98_RS01010 (HCM98_01010) 174602..176770 + 2169 WP_029439199.1 Tex family protein -
HCM98_RS01015 (HCM98_01015) 176828..176944 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HCM98_RS01020 (HCM98_01020) 177140..177598 + 459 WP_029439200.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T153547 WP_000635963.1 NZ_CP051013:174184-174534 [Bacillus tropicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T153547 NZ_CP066958:432899-433033 [Xanthomonas campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT153547 WP_000004570.1 NZ_CP051013:173892-174179 [Bacillus tropicus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT153547 NZ_CP066958:432670-432822 [Xanthomonas campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCTGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References