Detailed information of TA system TA153545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1236581..1237498
• Replicon: chromosome
• Accession: NZ_CP051011
• Organism: Bacillus velezensis strain BY6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HEQ83_RS06455
• Protein ID: WP_168237135.1
• Coordinates: 1236752..1237498 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HEQ83_RS06450
• Protein ID: WP_003154807.1
• Coordinates: 1236581..1236751 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEQ83_RS06415	1231813..1233435	+	1623	WP_069473361.1	pyocin knob domain-containing protein	-
HEQ83_RS06420	1233448..1233819	+	372	WP_168237132.1	hypothetical protein	-
HEQ83_RS06425	1233824..1234021	+	198	WP_168237133.1	XkdX family protein	-
HEQ83_RS06430	1234078..1234840	+	763	Protein_1200	phage portal protein	-
HEQ83_RS06435	1234892..1235155	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HEQ83_RS06440	1235169..1235432	+	264	WP_003154813.1	phage holin	-
HEQ83_RS06445	1235446..1236324	+	879	WP_168237134.1	N-acetylmuramoyl-L-alanine amidase	-
HEQ83_RS06450	1236581..1236751	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HEQ83_RS06455	1236752..1237498	-	747	WP_168237135.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HEQ83_RS06460	1237603..1238601	-	999	WP_003154805.1	inorganic phosphate transporter	-
HEQ83_RS06465	1238614..1239231	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HEQ83_RS06470	1239517..1240833	-	1317	WP_003154801.1	amino acid permease	-
HEQ83_RS06475	1241156..1242106	+	951	WP_015388352.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1189690..1273610	83920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29019.49 Da
• Isoelectric Point: 4.7698

>T153545 WP_168237135.1 NZ_CP051011:c1237498-1236752 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSDNLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T153545 NZ_CP066956:c4591981-4591829 [Xanthomonas campestris pv. incanae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCTGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT153545 WP_003154807.1 NZ_CP051011:c1236751-1236581 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT153545 NZ_CP066956:c4591752-4591618 [Xanthomonas campestris pv. incanae]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14