Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 475587..476224 Replicon chromosome
Accession NZ_CP051011
Organism Bacillus velezensis strain BY6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HEQ83_RS02455 Protein ID WP_003156187.1
Coordinates 475874..476224 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HEQ83_RS02450 Protein ID WP_003156188.1
Coordinates 475587..475868 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HEQ83_RS02430 471952..472551 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
HEQ83_RS02435 472644..473009 + 366 WP_003156192.1 holo-ACP synthase -
HEQ83_RS02440 473174..474181 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
HEQ83_RS02445 474298..475467 + 1170 WP_003156189.1 alanine racemase -
HEQ83_RS02450 475587..475868 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HEQ83_RS02455 475874..476224 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HEQ83_RS02460 476342..477163 + 822 WP_003156182.1 STAS domain-containing protein -
HEQ83_RS02465 477168..477533 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HEQ83_RS02470 477536..477937 + 402 WP_003156178.1 anti-sigma regulatory factor -
HEQ83_RS02475 477949..478956 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
HEQ83_RS02480 479020..479349 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HEQ83_RS02485 479346..479828 + 483 WP_003156173.1 anti-sigma B factor RsbW -
HEQ83_RS02490 479794..480582 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
HEQ83_RS02495 480582..481184 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T153544 WP_003156187.1 NZ_CP051011:475874-476224 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T153544 NZ_CP066956:c4591752-4591618 [Xanthomonas campestris pv. incanae]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT153544 WP_003156188.1 NZ_CP051011:475587-475868 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT153544 NZ_CP066956:c4591981-4591829 [Xanthomonas campestris pv. incanae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCTGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References