Detailed information of TA system TA153534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 2507949..2508644
• Replicon: chromosome
• Accession: NZ_CP051005
• Organism: Enterococcus faecalis strain TH4125

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: GRB94_RS12455
• Protein ID: WP_104802092.1
• Coordinates: 2508300..2508644 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: GRB94_RS12450
• Protein ID: WP_002373919.1
• Coordinates: 2507949..2508281 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRB94_RS12400	2503420..2504235	-	816	WP_161972151.1	PD-(D/E)XK nuclease-like domain-containing protein	-
GRB94_RS12405	2504198..2505163	-	966	WP_002415270.1	recombinase RecT	-
GRB94_RS12410	2505264..2505488	-	225	WP_104802087.1	hypothetical protein	-
GRB94_RS12415	2505485..2505784	-	300	WP_002388460.1	hypothetical protein	-
GRB94_RS12420	2505851..2506030	-	180	WP_104802088.1	hypothetical protein	-
GRB94_RS12425	2506065..2506334	-	270	WP_002365131.1	hypothetical protein	-
GRB94_RS12430	2506410..2506724	+	315	WP_010776102.1	hypothetical protein	-
GRB94_RS12435	2506980..2507162	+	183	WP_104802089.1	hypothetical protein	-
GRB94_RS12440	2507154..2507456	-	303	WP_104802090.1	hypothetical protein	-
GRB94_RS12445	2507469..2507651	-	183	WP_104802091.1	hypothetical protein	-
GRB94_RS12450	2507949..2508281	+	333	WP_002373919.1	helix-turn-helix domain-containing protein	Antitoxin
GRB94_RS12455	2508300..2508644	+	345	WP_104802092.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GRB94_RS12460	2508731..2508913	+	183	WP_010817602.1	hypothetical protein	-
GRB94_RS12465	2508915..2509562	-	648	WP_104802093.1	hypothetical protein	-
GRB94_RS12470	2509752..2510932	+	1181	Protein_2424	site-specific integrase	-
GRB94_RS12475	2511035..2511184	-	150	WP_002356321.1	50S ribosomal protein L33	-
GRB94_RS12480	2511310..2513445	-	2136	WP_002359399.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2468950..2510932	41982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13626.61 Da
• Isoelectric Point: 5.6177

>T153534 WP_104802092.1 NZ_CP051005:2508300-2508644 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMTLYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEVYLK

Nucleotide

• Length: 345 bp

>T153534 NZ_CP066949:c4707586-4707452 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12734.28 Da
• Isoelectric Point: 5.2423

>AT153534 WP_002373919.1 NZ_CP051005:2507949-2508281 [Enterococcus faecalis]
MNTYEIIKELTKRKKMSIRQLEITLGYSNGYFSKWKKVSPNSEGLQKVADYFNVSVDYLLGRTDNTKATDEKNSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT153534 NZ_CP066949:c4707815-4707663 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA