Detailed information of TA system TA153532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1502172..1503309
• Replicon: chromosome
• Accession: NZ_CP051005
• Organism: Enterococcus faecalis strain TH4125

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: GRB94_RS07455
• Protein ID: WP_002401483.1
• Coordinates: 1502446..1503309 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: GRB94_RS07450
• Protein ID: WP_000301765.1
• Coordinates: 1502172..1502444 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRB94_RS07415	1497678..1499165	+	1488	WP_010712965.1	type IA DNA topoisomerase	-
GRB94_RS07420	1499291..1499455	+	165	WP_002935077.1	peptide-binding protein	-
GRB94_RS07425	1499476..1499571	+	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
GRB94_RS07430	1499696..1500433	+	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
GRB94_RS07435	1500579..1500863	+	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
GRB94_RS07440	1500966..1501862	+	897	WP_002387620.1	ParA family protein	-
GRB94_RS07445	1501946..1502155	+	210	WP_000527317.1	peptide-binding protein	-
GRB94_RS07450	1502172..1502444	+	273	WP_000301765.1	antitoxin	Antitoxin
GRB94_RS07455	1502446..1503309	+	864	WP_002401483.1	zeta toxin family protein	Toxin
GRB94_RS07460	1503750..1504067	+	318	WP_002333463.1	hypothetical protein	-
GRB94_RS07465	1504087..1504374	+	288	Protein_1435	DnaJ domain-containing protein	-
GRB94_RS07470	1504448..1505128	+	681	WP_010710189.1	IS6-like element IS1216 family transposase	-
GRB94_RS07475	1505184..1505615	+	432	Protein_1437	2-dehydropantoate 2-reductase	-
GRB94_RS07480	1505891..1506742	+	852	WP_002357480.1	ribosome biogenesis GTPase YlqF	-
GRB94_RS07485	1506744..1507511	+	768	WP_002357481.1	ribonuclease HII	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III	-	1403193..1610866	207673
	-	inside	IScluster/Tn	erm(B) / aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III	-	1485854..1505128	19274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T153532 WP_002401483.1 NZ_CP051005:1502446-1503309 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T153532 NZ_CP066948:c4709163-4709011 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT153532 WP_000301765.1 NZ_CP051005:1502172-1502444 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT153532 NZ_CP066948:c4708934-4708800 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3