Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 392045..392579 Replicon chromosome
Accession NZ_CP051005
Organism Enterococcus faecalis strain TH4125

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag GRB94_RS01995 Protein ID WP_002360769.1
Coordinates 392304..392579 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag GRB94_RS01990 Protein ID WP_002369771.1
Coordinates 392045..392311 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRB94_RS01955 387903..388688 + 786 WP_002360778.1 replication-relaxation family protein -
GRB94_RS01960 388694..389182 + 489 WP_010710133.1 hypothetical protein -
GRB94_RS01965 389774..390040 + 267 WP_002377947.1 hypothetical protein -
GRB94_RS01970 390073..390573 + 501 WP_002360775.1 DnaJ domain-containing protein -
GRB94_RS01975 390586..390834 + 249 WP_002360773.1 DUF3850 domain-containing protein -
GRB94_RS01980 390910..391326 + 417 WP_010710134.1 single-stranded DNA-binding protein -
GRB94_RS01985 391350..391934 + 585 WP_002377949.1 thermonuclease family protein -
GRB94_RS01990 392045..392311 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GRB94_RS01995 392304..392579 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
GRB94_RS02000 392770..393450 - 681 Protein_369 IS6-like element IS1216 family transposase -
GRB94_RS02005 393535..394429 + 895 Protein_370 IS256 family transposase -
GRB94_RS02010 394734..395771 - 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
GRB94_RS02015 395797..396735 - 939 WP_002370935.1 2-dehydropantoate 2-reductase -
GRB94_RS02020 396863..397192 - 330 WP_002358661.1 LPXTG cell wall anchor domain-containing protein -
GRB94_RS02025 397222..397407 + 186 WP_002358660.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylM / cylB / cylA / cylI / esp 370955..431541 60586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T153531 WP_002360769.1 NZ_CP051005:392304-392579 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T153531 NZ_CP066948:c4708934-4708800 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT153531 WP_002369771.1 NZ_CP051005:392045-392311 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT153531 NZ_CP066948:c4709163-4709011 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References