153526

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4645865..4646448 Replicon chromosome
Accession NZ_CP051001
Organism Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag BY40_RS22950 Protein ID WP_000254738.1
Coordinates 4645865..4646200 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag BY40_RS22955 Protein ID WP_000581937.1
Coordinates 4646200..4646448 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BY40_RS22935 (4641752) 4641752..4643050 - 1299 WP_000036723.1 phosphopyruvate hydratase -
BY40_RS22940 (4643138) 4643138..4644775 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
BY40_RS22945 (4645003) 4645003..4645794 - 792 WP_001071661.1 nucleoside triphosphate pyrophosphohydrolase -
BY40_RS22950 (4645865) 4645865..4646200 - 336 WP_000254738.1 endoribonuclease MazF Toxin
BY40_RS22955 (4646200) 4646200..4646448 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
BY40_RS22960 (4646526) 4646526..4648760 - 2235 WP_000226815.1 GTP pyrophosphokinase -
BY40_RS22965 (4648808) 4648808..4650109 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T153526 WP_000254738.1 NZ_CP051001:c4646200-4645865 [Escherichia coli O157:H16 str. 98-3133]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T153526 NZ_CP066945:4920550-4920705 [Xanthomonas campestris pv. campestris]
GTGACCCTGCTCGATACCAATGTCATCTTCGGGCCCAATCTGCATGTCGTCGCCTGGATCGATGCGCAGGCCGTTGAAAC
ACTGTTCCTGTCGGTCGTGACGGTGGCCGAATTGCGATCTGGAGACATTCAGGATTTCAGACCCCTGATGAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT153526 WP_000581937.1 NZ_CP051001:c4646448-4646200 [Escherichia coli O157:H16 str. 98-3133]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT153526 NZ_CP066945:4920299-4920553 [Xanthomonas campestris pv. campestris]
ATGCCTTCTTTCACCGTCCGCAACATCCCGGAGGATGTGCATCGCGCCATCCGGGCACGTGCTGCCCAACACGGGCGCAG
CACCGAGGCGGAGATCCGCGCGATCCTTGAATCAGCAGCCAAGCCAGCCGGCCAGGTCAAACTGGGCGCGTTGCTGGTGG
ACATCGGCCGCGACGCTGGCCTGACAGCAAAAGAGGCCGACGCCTTCGCCAAGTTACGCGACAAGGCCGCGATCAAGCCG
ATCGGTTTGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References