Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2067003..2067621 Replicon chromosome
Accession NZ_CP051001
Organism Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BY40_RS10055 Protein ID WP_001291435.1
Coordinates 2067003..2067221 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BY40_RS10060 Protein ID WP_000344800.1
Coordinates 2067247..2067621 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BY40_RS10020 (2062292) 2062292..2062864 + 573 WP_000779831.1 YbaY family lipoprotein -
BY40_RS10025 (2062895) 2062895..2063206 - 312 WP_000409911.1 MGMT family protein -
BY40_RS10035 (2063585) 2063585..2063938 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BY40_RS10040 (2063980) 2063980..2065530 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BY40_RS10045 (2065694) 2065694..2066164 - 471 WP_000136192.1 YlaC family protein -
BY40_RS10050 (2066280) 2066280..2066831 - 552 WP_000102564.1 maltose O-acetyltransferase -
BY40_RS10055 (2067003) 2067003..2067221 - 219 WP_001291435.1 HHA domain-containing protein Toxin
BY40_RS10060 (2067247) 2067247..2067621 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BY40_RS10065 (2068167) 2068167..2071316 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
BY40_RS10070 (2071339) 2071339..2072532 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T153510 WP_001291435.1 NZ_CP051001:c2067221-2067003 [Escherichia coli O157:H16 str. 98-3133]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T153510 NZ_CP066936:c1234768-1234277 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCTTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT153510 WP_000344800.1 NZ_CP051001:c2067621-2067247 [Escherichia coli O157:H16 str. 98-3133]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT153510 NZ_CP066936:c1235055-1234765 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References