Detailed information of TA system TA153507

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1484519..1485351
• Replicon: chromosome
• Accession: NZ_CP051001
• Organism: Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: BY40_RS07270
• Protein ID: WP_000854753.1
• Coordinates: 1484977..1485351 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: BY40_RS07265
• Protein ID: WP_001315620.1
• Coordinates: 1484519..1484887 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BY40_RS07225 (1479868)	1479868..1479999	-	132	Protein_1409	IS3 family transposase	-
BY40_RS07230 (1480281)	1480281..1480472	+	192	Protein_1410	hypothetical protein	-
BY40_RS07235 (1480690)	1480690..1480815	-	126	Protein_1411	hemolysin activation protein	-
BY40_RS07240 (1481563)	1481563..1481769	+	207	Protein_1412	autotransporter adhesin family protein	-
BY40_RS07245 (1481890)	1481890..1483425	+	1536	WP_000939409.1	protein YeeR	-
BY40_RS07250 (1483410)	1483410..1483580	+	171	Protein_1414	antirestriction protein	-
BY40_RS07255 (1483596)	1483596..1484072	+	477	WP_001186774.1	RadC family protein	-
BY40_RS07260 (1484135)	1484135..1484356	+	222	WP_000692345.1	DUF987 domain-containing protein	-
BY40_RS07265 (1484519)	1484519..1484887	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BY40_RS07270 (1484977)	1484977..1485351	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
BY40_RS07275 (1485348)	1485348..1485836	+	489	WP_000777547.1	DUF5983 family protein	-
BY40_RS07280 (1485848)	1485848..1486045	+	198	WP_000839281.1	DUF957 domain-containing protein	-
BY40_RS07285 (1486130)	1486130..1486279	+	150	Protein_1421	restriction endonuclease subunit M	-
BY40_RS24160 (1487047)	1487047..1487190	-	144	Protein_1422	HNH endonuclease	-
BY40_RS07290 (1487327)	1487327..1487977	+	651	WP_001037966.1	HNH endonuclease	-
BY40_RS07295 (1487985)	1487985..1488965	-	981	WP_000991415.1	sialate O-acetylesterase	-
BY40_RS07300 (1489030)	1489030..1490136	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	1454383..1494004	39621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T153507 WP_000854753.1 NZ_CP051001:1484977-1485351 [Escherichia coli O157:H16 str. 98-3133]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T153507 NZ_CP066934:c4704590-4704456 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT153507 WP_001315620.1 NZ_CP051001:1484519-1484887 [Escherichia coli O157:H16 str. 98-3133]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT153507 NZ_CP066934:c4704819-4704667 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5