Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5734..6569 Replicon chromosome
Accession NZ_CP051001
Organism Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3A6S2N5
Locus tag BY40_RS00040 Protein ID WP_000854800.1
Coordinates 6192..6569 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3Z2YIX3
Locus tag BY40_RS00035 Protein ID WP_032178229.1
Coordinates 5734..6102 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BY40_RS00010 (2845) 2845..3663 + 819 WP_001234392.1 DUF932 domain-containing protein -
BY40_RS00015 (3755) 3755..4240 + 486 WP_000213706.1 antirestriction protein -
BY40_RS00020 (4255) 4255..4731 + 477 WP_001186784.1 RadC family protein -
BY40_RS00025 (4800) 4800..5021 + 222 WP_000692353.1 DUF987 domain-containing protein -
BY40_RS00030 (5040) 5040..5684 + 645 WP_000086748.1 hypothetical protein -
BY40_RS00035 (5734) 5734..6102 + 369 WP_032178229.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BY40_RS00040 (6192) 6192..6569 + 378 WP_000854800.1 TA system toxin CbtA family protein Toxin
BY40_RS00045 (6566) 6566..7054 + 489 WP_000779171.1 DUF5983 family protein -
BY40_RS00050 (7066) 7066..7263 + 198 WP_001374283.1 DUF957 domain-containing protein -
BY40_RS00055 (7348) 7348..8190 + 843 WP_001290183.1 DUF4942 domain-containing protein -
BY40_RS00060 (8471) 8471..9007 - 537 WP_000942785.1 GspM family type II secretion system protein YghD -
BY40_RS00065 (9051) 9051..10454 - 1404 Protein_12 inorganic phosphate transporter PitB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14068.13 Da        Isoelectric Point: 9.1376

>T153502 WP_000854800.1 NZ_CP051001:6192-6569 [Escherichia coli O157:H16 str. 98-3133]
MKTLPVLPGQAAGLRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDKPGF
NACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHSEAKR

Download         Length: 378 bp

>T153502 NZ_CP066932:c4561301-4561149 [Xanthomonas campestris pv. raphani]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13823.58 Da        Isoelectric Point: 6.3177

>AT153502 WP_032178229.1 NZ_CP051001:5734-6102 [Escherichia coli O157:H16 str. 98-3133]
VSDTLHETNYPDDNNDRSWWGLPCTVTPCFRARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT153502 NZ_CP066932:c4561069-4560935 [Xanthomonas campestris pv. raphani]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A6S2N5

Antitoxin

Source ID Structure
AlphaFold DB A0A3Z2YIX3

References