Detailed information of TA system TA153499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 34965..35566
• Replicon: plasmid pF8704-2_2
• Accession: NZ_CP050999
• Organism: Escherichia coli O39:NM str. F8704-2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A731GHK2
• Locus tag: P411_RS27290
• Protein ID: WP_001216038.1
• Coordinates: 34965..35345 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: P411_RS27295
• Protein ID: WP_001190712.1
• Coordinates: 35345..35566 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P411_RS27255	30845..31873	+	1029	WP_001292235.1	tyrosine-type recombinase/integrase	-
P411_RS27260	31947..32291	+	345	WP_001191776.1	hypothetical protein	-
P411_RS27265	32288..32764	+	477	WP_000861175.1	hypothetical protein	-
P411_RS27270	32761..33255	+	495	WP_000640907.1	dUTP diphosphatase	-
P411_RS27275	33270..33947	+	678	WP_001061874.1	DUF2829 domain-containing protein	-
P411_RS27280	33954..34730	+	777	WP_000432096.1	hypothetical protein	-
P411_RS27285	34763..34960	-	198	WP_000113017.1	hypothetical protein	-
P411_RS27290	34965..35345	-	381	WP_001216038.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
P411_RS27295	35345..35566	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
P411_RS27300	35675..36097	-	423	WP_000098854.1	hypothetical protein	-
P411_RS27305	36232..36621	-	390	WP_000506729.1	S24 family peptidase	-
P411_RS27310	36681..36779	-	99	Protein_40	DNA polymerase III subunit theta	-
P411_RS28535	36812..37162	+	351	WP_001436207.1	hypothetical protein	-
P411_RS27320	37147..37431	-	285	WP_001142397.1	hypothetical protein	-
P411_RS27325	37415..38266	-	852	WP_227456489.1	hypothetical protein	-
P411_RS28540	38447..38581	-	135	Protein_44	hypothetical protein	-
P411_RS27330	38578..38787	-	210	WP_000951712.1	hypothetical protein	-
P411_RS27335	38789..38983	-	195	WP_000224731.1	hypothetical protein	-
P411_RS27340	38985..39710	-	726	WP_000078034.1	ead/Ea22-like family protein	-
P411_RS27345	39707..39946	-	240	WP_000476201.1	hypothetical protein	-
P411_RS27350	39939..40142	-	204	WP_000158003.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74525	74525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T153499 WP_001216038.1 NZ_CP050999:c35345-34965 [Escherichia coli O39:NM str. F8704-2]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEISDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T153499 NZ_CP066931:c4774018-4773884 [Xanthomonas campestris pv. raphani]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT153499 WP_001190712.1 NZ_CP050999:c35566-35345 [Escherichia coli O39:NM str. F8704-2]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT153499 NZ_CP066931:c4774247-4774095 [Xanthomonas campestris pv. raphani]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: A0A731GHK2

Antitoxin

• AlphaFold DB: A0A829CJB6