Detailed information of TA system TA153490

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4916402..4917237
• Replicon: chromosome
• Accession: NZ_CP050998
• Organism: Escherichia coli O39:NM str. F8704-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A136X5A2
• Locus tag: P411_RS24655
• Protein ID: WP_000854757.1
• Coordinates: 4916402..4916779 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: P411_RS24660
• Protein ID: WP_024184487.1
• Coordinates: 4916869..4917237 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P411_RS24610 (4912025)	4912025..4912354	+	330	WP_000450409.1	DUF496 family protein	-
P411_RS28460 (4912455)	4912455..4912820	-	366	WP_001282181.1	EutP/PduV family microcompartment system protein	-
P411_RS24620 (4912832)	4912832..4912924	+	93	Protein_4743	ATP-binding protein	-
P411_RS24625 (4912946)	4912946..4914559	-	1614	WP_016236744.1	IS66-like element ISEc23 family transposase	-
P411_RS24630 (4914590)	4914590..4914940	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
P411_RS24635 (4914937)	4914937..4915362	-	426	WP_000422741.1	transposase	-
P411_RS24640 (4915447)	4915447..4915920	+	474	Protein_4747	IS21-like element ISEc12 family helper ATPase IstB	-
P411_RS24645 (4916076)	4916076..4916156	-	81	Protein_4748	hypothetical protein	-
P411_RS24650 (4916256)	4916256..4916405	-	150	Protein_4749	DUF5983 family protein	-
P411_RS24655 (4916402)	4916402..4916779	-	378	WP_000854757.1	TA system toxin CbtA family protein	Toxin
P411_RS24660 (4916869)	4916869..4917237	-	369	WP_024184487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
P411_RS24665 (4917400)	4917400..4917621	-	222	WP_000584198.1	DUF987 domain-containing protein	-
P411_RS24670 (4917690)	4917690..4918166	-	477	WP_001186770.1	RadC family protein	-
P411_RS24675 (4918182)	4918182..4918661	-	480	WP_000844095.1	antirestriction protein	-
P411_RS24680 (4918743)	4918743..4919561	-	819	WP_001234716.1	DUF932 domain-containing protein	-
P411_RS24685 (4919679)	4919679..4919874	-	196	Protein_4756	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14153.18 Da
• Isoelectric Point: 7.3249

>T153490 WP_000854757.1 NZ_CP050998:c4916779-4916402 [Escherichia coli O39:NM str. F8704-2]
MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T153490 NZ_CP066928:339360-339494 [Xanthomonas campestris pv. incanae]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13582.36 Da
• Isoelectric Point: 6.6255

>AT153490 WP_024184487.1 NZ_CP050998:c4917237-4916869 [Escherichia coli O39:NM str. F8704-2]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT153490 NZ_CP066928:339131-339283 [Xanthomonas campestris pv. incanae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCTGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: A0A136X5A2