153484

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3298314..3298900 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag P411_RS16635 Protein ID WP_042014162.1
Coordinates 3298314..3298544 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag P411_RS16640 Protein ID WP_000593555.1
Coordinates 3298541..3298900 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS16625 (3294351) 3294351..3297086 + 2736 WP_000149121.1 ribosome-associated ATPase/putative transporter RbbA -
P411_RS16630 (3297086) 3297086..3298210 + 1125 WP_001216257.1 ABC transporter permease -
P411_RS16635 (3298314) 3298314..3298544 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
P411_RS16640 (3298541) 3298541..3298900 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
P411_RS16645 (3299020) 3299020..3299421 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
P411_RS16650 (3299427) 3299427..3300233 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
P411_RS16655 (3300230) 3300230..3300994 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
P411_RS16660 (3300994) 3300994..3301827 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
P411_RS16665 (3301824) 3301824..3302768 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T153484 WP_042014162.1 NZ_CP050998:3298314-3298544 [Escherichia coli O39:NM str. F8704-2]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T153484 NZ_CP066925:355640-355774 [Xanthomonas campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT153484 WP_000593555.1 NZ_CP050998:3298541-3298900 [Escherichia coli O39:NM str. F8704-2]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT153484 NZ_CP066925:355411-355563 [Xanthomonas campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCTGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References