Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2504140..2504637 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag P411_RS12970 Protein ID WP_001173343.1
Coordinates 2504140..2504313 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag P411_RS12975 Protein ID WP_000398619.1
Coordinates 2504341..2504637 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS12960 (2500810) 2500810..2502267 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
P411_RS12965 (2502504) 2502504..2504021 + 1518 WP_001295091.1 lysine--tRNA ligase -
P411_RS12970 (2504140) 2504140..2504313 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
P411_RS12975 (2504341) 2504341..2504637 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
P411_RS12980 (2504864) 2504864..2505136 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
P411_RS12985 (2505148) 2505148..2505378 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
P411_RS12990 (2505559) 2505559..2507190 + 1632 WP_001216487.1 sensor histidine kinase -
P411_RS12995 (2507187) 2507187..2507906 + 720 WP_000611278.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T153482 WP_001173343.1 NZ_CP050998:c2504313-2504140 [Escherichia coli O39:NM str. F8704-2]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T153482 NZ_CP066923:4991471-4991626 [Xanthomonas campestris pv. incanae]
GTGACCCTGCTCGATACCAATGTCATCTTCGGGCCCAATCTGCATGTCGTCGCCTGGATCGATGTGCAGGCCGTTGAAAC
ACTGTTCCTGTCGGTCGTGACGGTGGCCGAATTGCGATCTGGAGACATTCAGGATTTCAGACCCCTGATGAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT153482 WP_000398619.1 NZ_CP050998:c2504637-2504341 [Escherichia coli O39:NM str. F8704-2]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT153482 NZ_CP066923:4991220-4991474 [Xanthomonas campestris pv. incanae]
ATGCCTTCTTTCACCGTCCGCAACATCCCGGAGGATGTGCATCGCGCCATCTGGGCACGTGCTGCCCAACACGGGCGCAG
CACCGAGGCGGAGATCCGCGCGATCCTTGAATCAGCAGCCAAGCCAGCCGACCAGGTCAAACTTGGCGCGTTGCTGGTGG
ACATCGGCCGCGACGCTGGCCTGACAGCAAAAGAGGCCGACGCCTTCGCCAAGTTACGCGACAAGGCCGCGATCAAGCCG
ATCGGTTTGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References