Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2264284..2264860 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag P411_RS11815 Protein ID WP_001299718.1
Coordinates 2264573..2264860 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag P411_RS11810 Protein ID WP_000063159.1
Coordinates 2264284..2264586 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS11795 (2260874) 2260874..2263023 + 2150 Protein_2243 pyruvate/proton symporter BtsT -
P411_RS11800 (2263073) 2263073..2263276 + 204 WP_000467859.1 YbdD/YjiX family protein -
P411_RS11805 (2263287) 2263287..2264243 + 957 WP_001299717.1 GTPase -
P411_RS11810 (2264284) 2264284..2264586 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
P411_RS11815 (2264573) 2264573..2264860 - 288 WP_001299718.1 BrnT family toxin Toxin
P411_RS11820 (2265057) 2265057..2266223 + 1167 Protein_2248 restriction endonuclease -
P411_RS11825 (2266287) 2266287..2267906 + 1620 WP_001029740.1 class I SAM-dependent DNA methyltransferase -
P411_RS11830 (2267896) 2267896..2269287 + 1392 WP_000535334.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD / espX6 2203651..2283912 80261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T153480 WP_001299718.1 NZ_CP050998:c2264860-2264573 [Escherichia coli O39:NM str. F8704-2]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T153480 NZ_CP050998:c2264860-2264573 [Escherichia coli O39:NM str. F8704-2]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT153480 WP_000063159.1 NZ_CP050998:c2264586-2264284 [Escherichia coli O39:NM str. F8704-2]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT153480 NZ_CP050998:c2264586-2264284 [Escherichia coli O39:NM str. F8704-2]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAATTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References