Detailed information of TA system TA153478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1726466..1727303
• Replicon: chromosome
• Accession: NZ_CP050998
• Organism: Escherichia coli O39:NM str. F8704-2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: P411_RS09165
• Protein ID: WP_000227784.1
• Coordinates: 1726466..1727008 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: P411_RS09170
• Protein ID: WP_001297137.1
• Coordinates: 1726992..1727303 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P411_RS09145 (1722005)	1722005..1722916	-	912	WP_000705865.1	2-dehydropantoate 2-reductase	-
P411_RS09150 (1723084)	1723084..1723575	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
P411_RS09155 (1723703)	1723703..1725067	-	1365	WP_001000978.1	MFS transporter	-
P411_RS09160 (1725475)	1725475..1726410	+	936	WP_001299550.1	tetratricopeptide repeat protein	-
P411_RS09165 (1726466)	1726466..1727008	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
P411_RS09170 (1726992)	1726992..1727303	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
P411_RS09175 (1727488)	1727488..1728378	-	891	WP_000971336.1	heme o synthase	-
P411_RS09180 (1728390)	1728390..1728719	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
P411_RS09185 (1728719)	1728719..1729333	-	615	WP_000179821.1	cytochrome o ubiquinol oxidase subunit III	-
P411_RS09190 (1729323)	1729323..1731314	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
P411_RS09195 (1731336)	1731336..1732283	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T153478 WP_000227784.1 NZ_CP050998:c1727008-1726466 [Escherichia coli O39:NM str. F8704-2]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T153478 NZ_CP066922:395011-395145 [Xanthomonas campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT153478 WP_001297137.1 NZ_CP050998:c1727303-1726992 [Escherichia coli O39:NM str. F8704-2]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT153478 NZ_CP066922:394779-394931 [Xanthomonas campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3