Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 386134..386772 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag P411_RS02350 Protein ID WP_000813794.1
Coordinates 386596..386772 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag P411_RS02345 Protein ID WP_001270286.1
Coordinates 386134..386550 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS02325 (381404) 381404..382227 - 824 Protein_381 ABC transporter permease -
P411_RS02330 (382228) 382228..383241 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
P411_RS02335 (383259) 383259..384404 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
P411_RS02340 (384649) 384649..386055 - 1407 WP_000760638.1 PLP-dependent aminotransferase family protein -
P411_RS02345 (386134) 386134..386550 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
P411_RS02350 (386596) 386596..386772 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
P411_RS02355 (386994) 386994..387224 + 231 WP_000494244.1 YncJ family protein -
P411_RS02360 (387316) 387316..389277 - 1962 WP_001298803.1 23S rRNA 5-hydroxycytidine C2501 synthase -
P411_RS02365 (389350) 389350..389886 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
P411_RS02370 (389978) 389978..391153 + 1176 WP_001236278.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 391193..392341 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T153475 WP_000813794.1 NZ_CP050998:c386772-386596 [Escherichia coli O39:NM str. F8704-2]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T153475 NZ_CP066921:c4608278-4608144 [Xanthomonas campestris pv. raphani]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT153475 WP_001270286.1 NZ_CP050998:c386550-386134 [Escherichia coli O39:NM str. F8704-2]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT153475 NZ_CP066921:c4608510-4608358 [Xanthomonas campestris pv. raphani]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References