Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 189386..189912 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag P411_RS01410 Protein ID WP_000323025.1
Coordinates 189625..189912 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag P411_RS01405 Protein ID WP_000534858.1
Coordinates 189386..189625 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS01380 (186190) 186190..186729 + 540 Protein_191 type III secretion system effector zinc metalloprotease NleC -
P411_RS01385 (186762) 186762..187016 - 255 Protein_192 transposase domain-containing protein -
P411_RS01390 (187073) 187073..188286 + 1214 WP_220541656.1 IS3 family transposase -
P411_RS01395 (188521) 188521..188853 - 333 WP_001317460.1 FlxA-like family protein -
P411_RS01400 (189056) 189056..189361 - 306 WP_001326990.1 protein YdfV -
P411_RS01405 (189386) 189386..189625 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
P411_RS01410 (189625) 189625..189912 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
P411_RS01415 (189984) 189984..190139 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
P411_RS01420 (190356) 190356..190607 + 252 WP_000980994.1 protein Rem -
P411_RS01425 (190674) 190674..190952 + 279 WP_012304870.1 hypothetical protein -
P411_RS01430 (190954) 190954..192003 + 1050 WP_001265198.1 DUF968 domain-containing protein -
P411_RS01435 (192017) 192017..192769 + 753 WP_001047137.1 antitermination protein -
P411_RS01440 (193047) 193047..193136 - 90 WP_120795389.1 hypothetical protein -
P411_RS01445 (193191) 193191..193403 - 213 WP_000087756.1 cold shock-like protein CspF -
P411_RS01450 (193704) 193704..193919 + 216 WP_000066484.1 cold shock-like protein CspB -
P411_RS01455 (194283) 194283..194453 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
P411_RS01460 (194673) 194673..194888 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleC 119263..237817 118554


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T153474 WP_000323025.1 NZ_CP050998:189625-189912 [Escherichia coli O39:NM str. F8704-2]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T153474 NZ_CP066919:2393187-2393321 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT153474 WP_000534858.1 NZ_CP050998:189386-189625 [Escherichia coli O39:NM str. F8704-2]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT153474 NZ_CP066919:2392958-2393110 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References