Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 45530..46077 Replicon plasmid pF8704-2_1
Accession NZ_CP050997
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag P411_RS00290 Protein ID WP_001384452.1
Coordinates 45799..46077 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q4FIL6
Locus tag P411_RS00285 Protein ID WP_000079945.1
Coordinates 45530..45799 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS00245 41178..41369 + 192 WP_071528006.1 hypothetical protein -
P411_RS00250 41613..42073 + 461 Protein_49 thermonuclease family protein -
P411_RS00255 42119..42328 + 210 WP_001298565.1 hemolysin expression modulator Hha -
P411_RS00260 42366..42956 + 591 WP_000766807.1 DUF2726 domain-containing protein -
P411_RS00265 43196..43444 + 249 WP_000083839.1 replication regulatory protein RepA -
P411_RS28160 43559..43739 + 181 Protein_53 protein CopA/IncA -
P411_RS00275 43758..44630 + 873 WP_220541634.1 incFII family plasmid replication initiator RepA -
P411_RS00280 45317..45457 + 141 WP_000829596.1 hypothetical protein -
P411_RS00285 45530..45799 + 270 WP_000079945.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
P411_RS00290 45799..46077 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
P411_RS00295 46149..46327 + 179 Protein_58 type II toxin-antitoxin system RelE/ParE family toxin -
P411_RS00300 46398..47279 + 882 WP_000005460.1 hypothetical protein -
P411_RS00305 47214..47633 - 420 WP_024181414.1 hypothetical protein -
P411_RS00310 47723..50727 - 3005 Protein_61 Tn3-like element Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(B) - 1..64010 64010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T153473 WP_001384452.1 NZ_CP050997:45799-46077 [Escherichia coli O39:NM str. F8704-2]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T153473 NZ_CP066919:2392958-2393110 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10180.52 Da        Isoelectric Point: 8.8735

>AT153473 WP_000079945.1 NZ_CP050997:45530-45799 [Escherichia coli O39:NM str. F8704-2]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT153473 NZ_CP066919:2393187-2393321 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB Q4FIL6

References