Detailed information of TA system TA153469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 696509..696956
• Replicon: plasmid unnamed
• Accession: NZ_CP050953
• Organism: Rhodococcus sp. DMU1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HFP48_RS31160
• Protein ID: WP_168581187.1
• Coordinates: 696509..696730 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HFP48_RS31165
• Protein ID: WP_168581112.1
• Coordinates: 696720..696956 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFP48_RS31140	693706..694950	+	1245	Protein_621	AAA family ATPase	-
HFP48_RS31145	695274..695417	+	144	WP_168581109.1	hypothetical protein	-
HFP48_RS31150	695449..695799	-	351	WP_168581110.1	hypothetical protein	-
HFP48_RS31155	695802..696260	-	459	WP_168581111.1	prevent-host-death protein	-
HFP48_RS31160	696509..696730	+	222	WP_168581187.1	BrnT family toxin	Toxin
HFP48_RS31165	696720..696956	+	237	WP_168581112.1	CopG family transcriptional regulator	Antitoxin
HFP48_RS31170	697054..697342	+	289	Protein_627	hypothetical protein	-
HFP48_RS31175	697737..699146	-	1410	WP_168581188.1	plasmid pRiA4b ORF-3 family protein	-
HFP48_RS31180	699191..699372	-	182	Protein_629	hypothetical protein	-
HFP48_RS31185	699819..700124	-	306	WP_168580683.1	alpha/beta hydrolase	-
HFP48_RS31190	700138..700842	-	705	WP_168581113.1	DUF899 family protein	-
HFP48_RS31195	701036..701302	+	267	WP_168581189.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..716018	716018
	-	flank	IS/Tn	-	-	701387..702340	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8375.57 Da
• Isoelectric Point: 10.2993

>T153469 WP_168581187.1 NZ_CP050953:696509-696730 [Rhodococcus sp. DMU1]
MAKHGIDFLSAQALWRDPHRLEVPAKTTDEPRWLVIGVIDGKWWSAVVTYRAGRTRIISVRRSRDGEVAPYES

Nucleotide

• Length: 222 bp

>T153469 NZ_CP066916:53208-53357 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9011.04 Da
• Isoelectric Point: 4.6702

>AT153469 WP_168581112.1 NZ_CP050953:696720-696956 [Rhodococcus sp. DMU1]
MRADEFDERFDAGEDVTFALDTARARRPGEEQRRVNVDFPAWMIAELDREATRLGVTRQSLIKVWIAERLERNGSTAT

Nucleotide

• Length: 237 bp

>AT153469 NZ_CP066916:c53163-53104 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT