Detailed information of TA system TA153468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 689920..690485
• Replicon: plasmid unnamed
• Accession: NZ_CP050953
• Organism: Rhodococcus sp. DMU1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HFP48_RS31130
• Protein ID: WP_168581107.1
• Coordinates: 690129..690485 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6H1CA67
• Locus tag: HFP48_RS31125
• Protein ID: WP_044476021.1
• Coordinates: 689920..690132 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFP48_RS31105	685464..685715	-	252	Protein_614	transposase	-
HFP48_RS31110	685809..687362	-	1554	WP_168581186.1	transposase	-
HFP48_RS31115	687451..688465	-	1015	Protein_616	IS256 family transposase	-
HFP48_RS31120	688702..689478	+	777	WP_168581106.1	VOC family protein	-
HFP48_RS31125	689920..690132	+	213	WP_044476021.1	ribbon-helix-helix domain-containing protein	Antitoxin
HFP48_RS31130	690129..690485	+	357	WP_168581107.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HFP48_RS31135	691311..693569	+	2259	WP_168581108.1	NADP-dependent isocitrate dehydrogenase	-
HFP48_RS31140	693706..694950	+	1245	Protein_621	AAA family ATPase	-
HFP48_RS31145	695274..695417	+	144	WP_168581109.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..716018	716018
	-	flank	IS/Tn	-	-	685464..685694	230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-40)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12864.57 Da
• Isoelectric Point: 8.9802

>T153468 WP_168581107.1 NZ_CP050953:690129-690485 [Rhodococcus sp. DMU1]
MIRRGHVHWVDLDPAQGSEAAKRRPAVVVSNDRANATAERLGRGVVTVVPLTSNTAHVFAFQVLVEPDESGMPRESKAQA
EQVRSVSVRRLDGDPVGKLSTRTLAALDDALRLHLDLH

Nucleotide

• Length: 357 bp

>T153468 NZ_CP066916:c6111-5695 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7602.32 Da
• Isoelectric Point: 4.0174

>AT153468 WP_044476021.1 NZ_CP050953:689920-690132 [Rhodococcus sp. DMU1]
MTTRFTISLPDEDARVLDEYVRDHGLGGRSAGIRAALRMLGEVGLERDYAAAFVEGGVEDDWDVTSADGE

Nucleotide

• Length: 213 bp

>AT153468 NZ_CP066916:c6338-6108 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6H1CA67