Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 428617..429158 Replicon plasmid unnamed
Accession NZ_CP050953
Organism Rhodococcus sp. DMU1

Toxin (Protein)


Gene name mazF Uniprot ID A0A6H1C8T2
Locus tag HFP48_RS29940 Protein ID WP_044475885.1
Coordinates 428859..429158 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6H1C8L3
Locus tag HFP48_RS29935 Protein ID WP_044475886.1
Coordinates 428617..428859 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFP48_RS29915 424636..425454 + 819 Protein_377 IS5 family transposase -
HFP48_RS29920 425883..426716 - 834 WP_168580949.1 alpha/beta fold hydrolase -
HFP48_RS29925 426713..427700 - 988 Protein_379 extradiol ring-cleavage dioxygenase -
HFP48_RS29930 428212..428610 - 399 Protein_380 hypothetical protein -
HFP48_RS29935 428617..428859 + 243 WP_044475886.1 antitoxin Antitoxin
HFP48_RS29940 428859..429158 + 300 WP_044475885.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HFP48_RS29945 429536..430180 - 645 WP_168580950.1 dihydrofolate reductase family protein -
HFP48_RS29950 430765..431924 - 1160 Protein_384 hypothetical protein -
HFP48_RS29955 431921..432271 - 351 WP_168580951.1 hypothetical protein -
HFP48_RS29960 432870..433766 + 897 WP_168580952.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..716018 716018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-95)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 10528.31 Da        Isoelectric Point: 10.3034

>T153467 WP_044475885.1 NZ_CP050953:428859-429158 [Rhodococcus sp. DMU1]
MRPIHAAKLDKTRPVLVLTRELVRPHLGRVTVAPITGTVRGLSTEVPVGPANGLKKDSVVSCDNIVTIPVSALGRSLGFF
FPAQEAALSEAIRAAFDLE

Download         Length: 300 bp

>T153467 NZ_CP066915:c5555705-5555322 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9017.12 Da        Isoelectric Point: 4.8950

>AT153467 WP_044475886.1 NZ_CP050953:428617-428859 [Rhodococcus sp. DMU1]
MSTQIAVRLPDEIVAFVDEEVREHRAPSRAALVLRALERERRRRIAARDVEILSRARGEADPDEFDGLARYAAGLSSDLD

Download         Length: 243 bp

>AT153467 NZ_CP066915:c5555947-5555705 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H1C8T2

Antitoxin

Source ID Structure
AlphaFold DB A0A6H1C8L3

References