Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5767194..5767825 Replicon chromosome
Accession NZ_CP050951
Organism Pseudomonas putida strain JQ581

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H5R9Z4
Locus tag A3L25_RS26240 Protein ID WP_063422462.1
Coordinates 5767598..5767825 (-) Length 76 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0C5S1B3
Locus tag A3L25_RS26235 Protein ID WP_016497905.1
Coordinates 5767194..5767601 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3L25_RS26215 5763730..5765178 + 1449 WP_063422458.1 phosphatase PAP2/dual specificity phosphatase family protein -
A3L25_RS26220 5765175..5765606 + 432 WP_063422460.1 hypothetical protein -
A3L25_RS26225 5765617..5766240 + 624 WP_016497906.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
A3L25_RS26230 5766242..5767174 + 933 WP_016489124.1 phosphatidate cytidylyltransferase -
A3L25_RS26235 5767194..5767601 - 408 WP_016497905.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A3L25_RS26240 5767598..5767825 - 228 WP_063422462.1 type II toxin-antitoxin system HicA family toxin Toxin
A3L25_RS26245 5768133..5768477 + 345 WP_003258412.1 cupin domain-containing protein -
A3L25_RS26250 5768687..5769574 - 888 WP_063422464.1 3-hydroxyisobutyrate dehydrogenase -
A3L25_RS26255 5769584..5771110 - 1527 WP_016497902.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -
A3L25_RS26260 5771243..5772193 + 951 WP_063422466.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-127)


Sequences

Toxin        


Download         Length: 76 a.a.        Molecular weight: 8250.39 Da        Isoelectric Point: 10.8172

>T153466 WP_063422462.1 NZ_CP050951:c5767825-5767598 [Pseudomonas putida]
MRSREVIQLIEADGWYEVEVKGSHQQFRHPTKKGRVTVPHPKTELPKGTVRSILKQAGLIQTGSVASTSVNGGNQ

Download         Length: 228 bp

>T153466 NZ_CP066915:c5232188-5231271 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14637.49 Da        Isoelectric Point: 4.7269

>AT153466 WP_016497905.1 NZ_CP050951:c5767601-5767194 [Pseudomonas putida]
MKIPVVLHKDTGSDYGVTVPDVPGCFSAGATVAQALENVQEALALHFEGLVADNEPLPQAQEVDVHVANPDYEGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELALSH

Download         Length: 408 bp

>AT153466 NZ_CP066915:c5232952-5232194 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5R9Z4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5S1B3

References