Detailed information of TA system TA153464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 4171739..4172622
• Replicon: chromosome
• Accession: NZ_CP050951
• Organism: Pseudomonas putida strain JQ581

Toxin (Protein)

• Gene name: -
• Uniprot ID: R9VG37
• Locus tag: A3L25_RS18890
• Protein ID: WP_016487683.1
• Coordinates: 4171739..4172176 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R9VFG2
• Locus tag: A3L25_RS18895
• Protein ID: WP_016487684.1
• Coordinates: 4172173..4172622 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3L25_RS18870	4166917..4167981	+	1065	WP_063426839.1	TerC family protein	-
A3L25_RS18875	4168087..4169316	-	1230	WP_063426841.1	acyl-CoA dehydrogenase	-
A3L25_RS18880	4169456..4170382	+	927	WP_063426843.1	LysR family transcriptional regulator	-
A3L25_RS18885	4170577..4171731	+	1155	WP_063426845.1	aminotransferase class V-fold PLP-dependent enzyme	-
A3L25_RS18890	4171739..4172176	-	438	WP_016487683.1	RES family NAD+ phosphorylase	Toxin
A3L25_RS18895	4172173..4172622	-	450	WP_016487684.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
A3L25_RS18900	4172729..4173505	-	777	WP_063426847.1	SDR family oxidoreductase	-
A3L25_RS18905	4173676..4174596	-	921	WP_059396273.1	LysR family transcriptional regulator	-
A3L25_RS18910	4174910..4175395	+	486	WP_063426848.1	transposase	-
A3L25_RS18915	4175495..4176217	-	723	WP_063426850.1	hypothetical protein	-
A3L25_RS18920	4176297..4176596	-	300	WP_063426851.1	DUF3077 domain-containing protein	-
A3L25_RS18925	4176795..4177418	+	624	WP_049274700.1	glutathione S-transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16005.35 Da
• Isoelectric Point: 6.2187

>T153464 WP_016487683.1 NZ_CP050951:c4172176-4171739 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPSGAMLEVLVHLEIDPEDFPTTMRLIRIELPDTVSQAQLPALQ
PGWSAQPELTRALGNRFLDDCSALLLPVPSAIMPSTINYLFNPRHAQAQRAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T153464 NZ_CP066915:c4669396-4668989 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17096.64 Da
• Isoelectric Point: 6.1089

>AT153464 WP_016487684.1 NZ_CP050951:c4172622-4172173 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLEIPELASDFEIHTRITHGFAATWLVKLTERGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEADKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT153464 NZ_CP066915:c4669643-4669377 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: R9VG37

Antitoxin

• AlphaFold DB: R9VFG2