Detailed information of TA system TA153462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 373648..374516
• Replicon: chromosome
• Accession: NZ_CP050951
• Organism: Pseudomonas putida strain JQ581

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A3L25_RS01620
• Protein ID: WP_169725174.1
• Coordinates: 373648..374001 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H5QWQ0
• Locus tag: A3L25_RS01625
• Protein ID: WP_063423236.1
• Coordinates: 374043..374516 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3L25_RS01605	368921..370288	-	1368	WP_063423239.1	HAMP domain-containing protein	-
A3L25_RS01610	370290..371012	-	723	WP_063423233.1	response regulator	-
A3L25_RS01615	371153..373450	+	2298	WP_063423234.1	TonB-dependent siderophore receptor	-
A3L25_RS01620	373648..374001	+	354	WP_169725174.1	type II toxin-antitoxin system HigB family toxin	Toxin
A3L25_RS01625	374043..374516	+	474	WP_063423236.1	hypothetical protein	Antitoxin
A3L25_RS01630	375162..376865	+	1704	WP_081237857.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13702.91 Da
• Isoelectric Point: 10.6182

>T153462 WP_169725174.1 NZ_CP050951:373648-374001 [Pseudomonas putida]
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPTSRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 354 bp

>T153462 NZ_CP066915:2745763-2747085 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17330.78 Da
• Isoelectric Point: 4.4982

>AT153462 WP_063423236.1 NZ_CP050951:374043-374516 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMTLIDELYAHADTEGHAAARFAEMVADR
VYEYESESVWVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT153462 NZ_CP066915:2745497-2745763 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H5QWQ0