Detailed information of TA system TA153460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 130451..131021
• Replicon: plasmid Ps2192A-p7
• Accession: NZ_CP050945
• Organism: Piscirickettsia salmonis strain Ps-2192A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GW536_RS18990
• Protein ID: WP_036781349.1
• Coordinates: 130451..130777 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GW536_RS18995
• Protein ID: WP_016212365.1
• Coordinates: 130779..131021 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW536_RS18955	125582..125839	-	258	Protein_152	DDE-type integrase/transposase/recombinase	-
GW536_RS18960	126127..126504	+	378	Protein_153	DDE-type integrase/transposase/recombinase	-
GW536_RS18965	126605..127384	+	780	WP_081377351.1	transposase	-
GW536_RS18970	127573..127905	+	333	WP_155047283.1	transposase	-
GW536_RS18975	128070..128447	+	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
GW536_RS18980	128754..129137	-	384	WP_016212152.1	hypothetical protein	-
GW536_RS18985	129607..130335	-	729	WP_054300202.1	IS6 family transposase	-
GW536_RS18990	130451..130777	-	327	WP_036781349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GW536_RS18995	130779..131021	-	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
GW536_RS19000	131226..131666	+	441	Protein_161	tyrosine-type recombinase/integrase	-
GW536_RS19005	131734..132462	+	729	WP_075274745.1	IS6-like element ISPsa2 family transposase	-
GW536_RS19010	132745..134328	+	1584	WP_052047129.1	protein kinase	-
GW536_RS19015	134449..135180	+	732	WP_016212311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..212888	212888
	-	inside	IScluster/Tn	-	-	118173..136068	17895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11971.89 Da
• Isoelectric Point: 10.1733

>T153460 WP_036781349.1 NZ_CP050945:c130777-130451 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T153460 NZ_CP066915:717559-718092 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT153460 WP_016212365.1 NZ_CP050945:c131021-130779 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT153460 NZ_CP066915:717282-717548 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA