Detailed information of TA system TA153459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 39730..40336
• Replicon: plasmid Ps2192A-p3
• Accession: NZ_CP050941
• Organism: Piscirickettsia salmonis strain Ps-2192A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: GW536_RS17805
• Protein ID: WP_027242955.1
• Coordinates: 39730..39990 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GW536_RS17810
• Protein ID: WP_036780061.1
• Coordinates: 39983..40336 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW536_RS17790	35960..36985	+	1026	WP_032126388.1	helix-turn-helix domain-containing protein	-
GW536_RS17795	37098..38330	+	1233	WP_032126623.1	MFS transporter	-
GW536_RS17800	38507..39481	-	975	WP_054300271.1	IS30 family transposase	-
GW536_RS17805	39730..39990	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
GW536_RS17810	39983..40336	+	354	WP_036780061.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GW536_RS17815	40613..41341	+	729	WP_017375910.1	IS6 family transposase	-
GW536_RS17820	41408..41698	-	291	Protein_60	IS6 family transposase	-
GW536_RS17825	41728..42660	-	933	WP_144420837.1	hypothetical protein	-
GW536_RS17830	42802..43530	-	729	WP_017375910.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66586	66586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T153459 WP_027242955.1 NZ_CP050941:39730-39990 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T153459 NZ_CP066915:613315-613599 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.44 Da
• Isoelectric Point: 5.0200

>AT153459 WP_036780061.1 NZ_CP050941:39983-40336 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQIVKR

Nucleotide

• Length: 354 bp

>AT153459 NZ_CP066915:613083-613325 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87