Detailed information of TA system TA153458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 18598..19241
• Replicon: plasmid Ps2192A-p1
• Accession: NZ_CP050939
• Organism: Piscirickettsia salmonis strain Ps-2192A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: GW536_RS17245
• Protein ID: WP_016210974.1
• Coordinates: 18834..19241 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: GW536_RS17240
• Protein ID: WP_016210972.1
• Coordinates: 18598..18825 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW536_RS17195	14232..14792	+	561	WP_016210973.1	hypothetical protein	-
GW536_RS17200	14761..15123	+	363	WP_016210959.1	hypothetical protein	-
GW536_RS17205	15116..15463	+	348	WP_155047331.1	hypothetical protein	-
GW536_RS17210	15460..15699	+	240	WP_155047325.1	hypothetical protein	-
GW536_RS17215	15692..16081	+	390	WP_036780969.1	hypothetical protein	-
GW536_RS17220	16078..16362	+	285	WP_036794070.1	hypothetical protein	-
GW536_RS17225	16364..16837	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
GW536_RS17230	16979..17308	+	330	WP_155047330.1	hypothetical protein	-
GW536_RS17235	17328..18302	-	975	WP_054300271.1	IS30 family transposase	-
GW536_RS17240	18598..18825	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GW536_RS17245	18834..19241	+	408	WP_016210974.1	DUF4411 family protein	Toxin
GW536_RS17250	19253..20038	+	786	WP_155047324.1	DNA/RNA non-specific endonuclease	-
GW536_RS17255	20467..20793	+	327	WP_155047323.1	zinc ribbon domain-containing protein	-
GW536_RS17260	20793..21122	+	330	WP_036780870.1	hypothetical protein	-
GW536_RS17265	21275..21640	+	366	WP_016212235.1	hypothetical protein	-
GW536_RS17270	21738..22712	+	975	WP_054300271.1	IS30 family transposase	-
GW536_RS17275	23047..23439	+	393	WP_155047322.1	HNH endonuclease	-
GW536_RS17280	23516..23995	+	480	WP_016212234.1	P27 family phage terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49531	49531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T153458 WP_016210974.1 NZ_CP050939:18834-19241 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T153458 NZ_CP066915:c476851-476564 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT153458 WP_016210972.1 NZ_CP050939:18598-18825 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT153458 NZ_CP066915:c477090-476851 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5