Detailed information of TA system TA153436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 2038292..2038898
• Replicon: chromosome
• Accession: NZ_CP050869
• Organism: Saccharolobus solfataricus strain POZ149

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q97UE3
• Locus tag: HFC64_RS10695
• Protein ID: WP_009989090.1
• Coordinates: 2038292..2038696 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UBQ9
• Locus tag: HFC64_RS10700
• Protein ID: WP_009989091.1
• Coordinates: 2038677..2038898 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC64_RS10665 (HFC64_10605)	2033794..2034555	-	762	WP_009990867.1	ZIP family metal transporter	-
HFC64_RS10670 (HFC64_10610)	2034703..2034885	-	183	WP_009990866.1	hypothetical protein	-
HFC64_RS10675 (HFC64_10620)	2035721..2036500	+	780	WP_014511459.1	DMT family transporter	-
HFC64_RS10680 (HFC64_10625)	2036938..2037662	+	725	Protein_2150	transposase	-
HFC64_RS10685 (HFC64_10630)	2037660..2037767	-	108	Protein_2151	IS110 family transposase	-
HFC64_RS10690 (HFC64_10635)	2037809..2038096	+	288	WP_272898411.1	transposase	-
HFC64_RS10695 (HFC64_10640)	2038292..2038696	-	405	WP_009989090.1	PIN domain-containing protein	Toxin
HFC64_RS10700 (HFC64_10645)	2038677..2038898	-	222	WP_009989091.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HFC64_RS10705 (HFC64_10650)	2039147..2039287	+	141	WP_010924124.1	hypothetical protein	-
HFC64_RS10710 (HFC64_10655)	2039427..2039627	+	201	WP_009989095.1	hypothetical protein	-
HFC64_RS10715 (HFC64_10660)	2039681..2039911	+	231	WP_009989096.1	EamA family transporter	-
HFC64_RS10720 (HFC64_10665)	2040022..2041293	+	1272	WP_009989097.1	MFS transporter	-
HFC64_RS10725 (HFC64_10670)	2041412..2042077	+	666	WP_009989098.1	helix-turn-helix domain-containing protein	-
HFC64_RS10730 (HFC64_10675)	2042074..2042460	-	387	WP_009989099.1	hypothetical protein	-
HFC64_RS10735 (HFC64_10680)	2042457..2043074	-	618	WP_009989100.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (4-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15426.98 Da
• Isoelectric Point: 5.0272

>T153436 WP_009989090.1 NZ_CP050869:c2038696-2038292 [Saccharolobus solfataricus]
VEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNV
TDAIGVLNKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS

Nucleotide

• Length: 405 bp

>T153436 NZ_CP066909:2792-3058 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8267.50 Da
• Isoelectric Point: 9.6908

>AT153436 WP_009989091.1 NZ_CP050869:c2038898-2038677 [Saccharolobus solfataricus]
MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS

Nucleotide

• Length: 222 bp

>AT153436 NZ_CP066909:2584-2808 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E3K068

Antitoxin

• AlphaFold DB: M9UBQ9