Detailed information of TA system TA153432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 685258..685905
• Replicon: chromosome
• Accession: NZ_CP050869
• Organism: Saccharolobus solfataricus strain POZ149

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HFC64_RS03470
• Protein ID: WP_009990357.1
• Coordinates: 685258..685530 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E3K976
• Locus tag: HFC64_RS03475
• Protein ID: WP_009990356.1
• Coordinates: 685645..685905 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC64_RS03455 (HFC64_03410)	680573..681624	-	1052	Protein_698	ISH3-like element ISC1359 family transposase	-
HFC64_RS03460 (HFC64_03415)	681728..682477	+	750	Protein_699	GDP-mannose 4,6-dehydratase	-
HFC64_RS03465 (HFC64_03420)	683788..684735	-	948	WP_009990359.1	ATP/GTP-binding protein	-
HFC64_RS03470 (HFC64_03430)	685258..685530	-	273	WP_009990357.1	PIN domain-containing protein	Toxin
HFC64_RS03475 (HFC64_03435)	685645..685905	-	261	WP_009990356.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HFC64_RS03480 (HFC64_03440)	686592..686753	+	162	WP_009990355.1	hypothetical protein	-
HFC64_RS03485 (HFC64_03445)	686855..687313	+	459	WP_009990354.1	hypothetical protein	-
HFC64_RS03490 (HFC64_03450)	688062..688223	-	162	Protein_705	TQO small subunit DoxA domain-containing protein	-
HFC64_RS03495 (HFC64_03455)	688303..689262	+	960	WP_010923364.1	IS5-like element ISC1234 family transposase	-
HFC64_RS03500 (HFC64_03460)	689435..689800	-	366	Protein_707	TQO small subunit DoxA domain-containing protein	-
HFC64_RS03505 (HFC64_03465)	689806..690348	-	543	WP_009990352.1	thiosulfate:quinone oxidoreductase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	669117..697334	28217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (20-82)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10475.34 Da
• Isoelectric Point: 9.7534

>T153432 WP_009990357.1 NZ_CP050869:c685530-685258 [Saccharolobus solfataricus]
MRLFTLSKKKYNVKFEDTIDFLDKIILPYTIILPINSHDYEKAKGIMISKTLKPSDAFHVAVMINNSIKKIVSEDSDFDK
INGIERIWVK

Nucleotide

• Length: 273 bp

>T153432 NZ_CP066905:c98132-97689 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9842.51 Da
• Isoelectric Point: 5.2456

>AT153432 WP_009990356.1 NZ_CP050869:c685905-685645 [Saccharolobus solfataricus]
MTLKIEVGKKGYIIIPKTVRDLLEIKEGDILILRVEDGKIVLEPERKVSFDDLKKKMEEHSAKISYANKAKLGDLINVSL
EEEFDN

Nucleotide

• Length: 261 bp

>AT153432 NZ_CP066905:c98359-98129 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E3K976