Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 196957..197482 Replicon plasmid unnamed1
Accession NZ_CP050866
Organism Escherichia coli strain 8-3-Ti3

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag HFC66_RS26095 Protein ID WP_001159860.1
Coordinates 197177..197482 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag HFC66_RS26090 Protein ID WP_000813626.1
Coordinates 196957..197175 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC66_RS26050 192185..192469 + 285 WP_038341659.1 ribbon-helix-helix domain-containing protein -
HFC66_RS26055 192469..192744 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
HFC66_RS26450 192876..193013 - 138 WP_000766699.1 hypothetical protein -
HFC66_RS26065 193120..193471 - 352 Protein_231 plasmid partitioning/stability family protein -
HFC66_RS26070 193474..194042 - 569 Protein_232 plasmid segregation protein ParM -
HFC66_RS26075 194050..195141 + 1092 Protein_233 IS66 family transposase -
HFC66_RS26080 195336..196544 - 1209 Protein_234 IS91 family transposase -
HFC66_RS26085 196525..196797 - 273 WP_000019158.1 hypothetical protein -
HFC66_RS26090 196957..197175 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HFC66_RS26095 197177..197482 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
HFC66_RS26100 197518..197613 + 96 Protein_238 transposase domain-containing protein -
HFC66_RS26105 197875..199512 + 1638 WP_039060807.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
HFC66_RS26110 199720..199989 + 270 WP_000079956.1 hypothetical protein -
HFC66_RS26455 199986..200212 + 227 Protein_241 type II toxin-antitoxin system RelE/ParE family toxin -
HFC66_RS26115 200241..200726 + 486 WP_038339729.1 protein kinase -
HFC66_RS26120 200741..201438 - 698 Protein_243 IS1 family transposase -
HFC66_RS26125 201337..201552 + 216 WP_167839519.1 hypothetical protein -
HFC66_RS26460 201562..201753 - 192 Protein_245 IS66 family insertion sequence element accessory protein TnpB -
HFC66_RS26130 201751..202075 + 325 Protein_246 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / senB / faeF / faeH / faeI / ospC1 / ospD3/senA / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..233800 233800
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 180698..201244 20546


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T153430 WP_001159860.1 NZ_CP050866:197177-197482 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T153430 NZ_CP066905:c12176-11865 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT153430 WP_000813626.1 NZ_CP050866:196957-197175 [Escherichia coli]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT153430 NZ_CP066905:c11868-11449 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References